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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1976-12-30
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pubmed:abstractText |
1. Sodium transport across isolated frog skin, as measured by the short-circuit current, was decreased by acetylsalicylic acid, mefenamic acid, paracetamol and phenylbutazone. Indomethacin (6 X 10(-6) M) had a biphasic effect on the short-circuit current: a transient increase followed by a sustained decrease. 2. The release of prostaglandin-like material from the skin was reduced by acetylsalicylic acid and indomethacin. Paracetamol caused a significant reduction in the short-circuit current response of the skin to low doses of arachidonic acid, but the response to the highest dose tested was not significantly altered. 3. Indomethacin (6 X 10(-6) M) increased the sensitivity of the skin to applied prostaglandin E1. The other prostaglandin synthetase inhibitors did not have this effect. Indomethacin (6 X 10(-6) M) also enhanced the effect of antidiuretic hormone on the short-circuit current. 4. Indomethacin (30 X 10(-6) M) increased the short-circuit current and diminished the response to applied prostaglandin E1. 5. In sulphate Ringer, theophylline increased the short-circuit current and diminished the response to prostaglandin E1. 6. Prostaglandin E1 increased the levels of cyclic AMP in frog skin and these increases preceded the increases in short-circuit current. There was a seasonal variation in the level of cyclic AMP in the skin: the levels in winter exceeded those in summer. There was also a seasonal variation in the cyclic AMP response to prostaglandin E1: the winter response was greater than that in summer. 7. Indomethacin (6 X 10(-6) M) had a biphasic effect on cyclic AMP levels in the skin, an initial increase followed by a decrease. Indomethacin also potentiated prostaglandin E1 stimulated cyclic AMP accumulation. 8. Theophylline increased cyclic AMP levels in the skin and potentiated prostaglandin E1 stimulated cyclic AMP accumulation. 9. Pre-treatment of the skin with theophylline reversed the effects of cyclic AMP on the short-circuit current and open-circuit potential. 10. It is concluded that endogenous prostaglandins help to maintain sodium transport across isolated frog skin and that the effects of E-type prostaglandins on the short-circuit current are mediated by increased cyclic AMP levels. The transient increase in short-circuit current and the increased skin sensitivity caused by indomethacin (6 X 10(-6) M) are attributed to inhibition of phosphodiesterase activity. The failure of theophylline to potentiate the short-circuit current response of the skin to prostaglandin E1 is attributed to alteration of cyclic AMP action on the skin by theophylline.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-13460242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-14013785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-163198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-166165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4203719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4237758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4242274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4303528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4304235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4306981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4310585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4319349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4323900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4330379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4342210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4345079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4368716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4371248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4385510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4386542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4396972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4505422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4544801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4547193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-4755427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-5072227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-5085240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-5183284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-5284360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-5284361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-5284362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-5558256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-5656636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-5689289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-5761999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-5768132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-5845852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/185363-6074965
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
258
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
731-53
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:185363-Acetaminophen,
pubmed-meshheading:185363-Animals,
pubmed-meshheading:185363-Arachidonic Acids,
pubmed-meshheading:185363-Biological Transport,
pubmed-meshheading:185363-Cyclic AMP,
pubmed-meshheading:185363-Female,
pubmed-meshheading:185363-Indomethacin,
pubmed-meshheading:185363-Male,
pubmed-meshheading:185363-Prostaglandins E,
pubmed-meshheading:185363-Rana temporaria,
pubmed-meshheading:185363-Skin,
pubmed-meshheading:185363-Sodium,
pubmed-meshheading:185363-Theophylline
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pubmed:year |
1976
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pubmed:articleTitle |
Endogenous prostaglandins, adenosine 3':5'-monophosphate and sodium transport across isolated frog skin.
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pubmed:publicationType |
Journal Article,
In Vitro
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