Linked Life Data

18534694

Source:http://linkedlifedata.com/resource/pubmed/id/18534694

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-6-23
pubmed:abstractText
This paper describes the development and validation of a PCR array for studying chemical-induced effects on gene expression of selected endocrine pathways along the hypothalamic-pituitary-gonadal (HPG) axis of the small, oviparous fish, the Japanese medaka (Oryzias latipes). The Japanese medaka HPG-PCR array combines the quantitative performance of SYBR Green-based real-time PCR with the multiple gene profiling capabilities of a microarray to examine expression profiles of 36 genes associated with endocrine pathways in brain, liver and gonad. The performance of the Japanese medaka HPG-PCR array was evaluated by examining effects of two model compounds, the synthetic estrogen, 17alpha-ethinylestradiol (EE2) and the anabolic androgen, 17beta-trenbolone (TRB) on the HPG axis of the Japanese medaka. Four-month-old medaka was exposed to three concentrations of EE2 (5, 50, 500 ng/L) or TRB (50, 500, 5000 ng/L) for 7d in a static renewal exposure system. A pathway-based approach was implemented to analyze and visualize concentration-dependent mRNA expression in the HPG axis of Japanese medaka. The compensatory response to EE2 exposure included the down-regulation of male brain GnRH RI and testicular CYP17. The down-regulation of AR-alpha expression in brain of EE2-exposed males was associated with suppression of male sexual behavior. Compensatory responses to TRB in the female HPG axis included up-regulation of brain GnRH RII and ovary steroidogenic CYP19A. Overall, the results suggested that the Japanese medaka HPG-PCR array has potential not only as a screening tool of potential endocrine-disrupting chemicals but also in elucidating mechanisms of action.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0166-445X
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
173-82
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:18534694-Animals, pubmed-meshheading:18534694-Aromatase, pubmed-meshheading:18534694-Ethinyl Estradiol, pubmed-meshheading:18534694-Female, pubmed-meshheading:18534694-Gene Expression, pubmed-meshheading:18534694-Gene Expression Profiling, pubmed-meshheading:18534694-Hypothalamo-Hypophyseal System, pubmed-meshheading:18534694-Male, pubmed-meshheading:18534694-Oryzias, pubmed-meshheading:18534694-Pituitary-Adrenal System, pubmed-meshheading:18534694-RNA, Messenger, pubmed-meshheading:18534694-Receptors, Androgen, pubmed-meshheading:18534694-Receptors, LHRH, pubmed-meshheading:18534694-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:18534694-Statistics, Nonparametric, pubmed-meshheading:18534694-Steroid 17-alpha-Hydroxylase, pubmed-meshheading:18534694-Trenbolone Acetate
pubmed:year
2008
pubmed:articleTitle
Real-time PCR array to study effects of chemicals on the Hypothalamic-Pituitary-Gonadal axis of the Japanese medaka.
pubmed:affiliation
Department of Zoology, National Food Safety & Toxicology Center, Michigan State University, East Lansing, 218c, MI 48824, USA. howard50003250@yahoo.com <howard50003250@yahoo.com>
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't