Source:http://linkedlifedata.com/resource/pubmed/id/18528549
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2008-6-5
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pubmed:abstractText |
We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1474-905X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
668-70
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pubmed:meshHeading |
pubmed-meshheading:18528549-Fluorescence,
pubmed-meshheading:18528549-Green Fluorescent Proteins,
pubmed-meshheading:18528549-HeLa Cells,
pubmed-meshheading:18528549-Humans,
pubmed-meshheading:18528549-Hydrogen-Ion Concentration,
pubmed-meshheading:18528549-Microscopy, Fluorescence,
pubmed-meshheading:18528549-Molecular Structure,
pubmed-meshheading:18528549-Time Factors
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pubmed:year |
2008
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pubmed:articleTitle |
Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements.
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pubmed:affiliation |
Research Institute for Electronic Science, Hokkaido University, Sapporo, 060-0812, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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