Source:http://linkedlifedata.com/resource/pubmed/id/18524490
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2008-6-10
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| pubmed:abstractText |
Activation of serotonin (5-hydroxytryptamine, 5-HT) receptors produces various autonomic and neuroendocrine responses in the hypothalamic paraventricular nucleus (PVN), including increased blood pressure and heart rate. However, the role(s) of 5-HT on the local GABA synaptic circuit have not been well understood in the PVN, where the inhibitory neurotransmitter GABA plays a key role in the modulation of sympathoexcitatory outflow. In the present study, we examined the effects of 5-HT on GABA synaptic transmission in presympathetic PVN neurons projecting to spinal cord using patch-clamp electrophysiology combined with tract-tracing techniques. Bath application of 5-HT (0.01-100 microM) reversibly decreased the frequency of spontaneous GABAergic inhibitory postsynaptic currents (sIPSC) in a concentration dependent manner (IC50, 0.07 microM), with no significant changes in the amplitudes and decay kinetics of sIPSC. The sIPSC inhibition of 5-HT was mimicked by 5-HT1A agonist, 8-OH-DPAT (8-hydroxy-2(di-n-propylamino)tetralin, 10 microM), and blocked by 5-HT1A antagonist WAY-100635 but not by 5-HT1B antagonist SB224289. 5-HT also reduced the frequency of miniature IPSC (mIPSC) (2.59+/-0.51 Hz, control vs. 1.25+/-0.31 Hz, 5-HT, n=16) in similar extent with 5-HT induced reduction of sIPSC frequency (sIPSCs, 55.8+/-6.2%, n=11 vs. mIPSCs, 52.30+/-5.85%, n=16; p>0.5). All together, our results indicate that 5-HT can inhibit presynaptic GABA release via presynaptic 5-HT1A receptors in presympathetic PVN neurons projecting to spinal cord.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/8-Hydroxy-2-(di-n-propylamino)tetral...,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/Piperidones,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/SB 22489G,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Spiro Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/WAY 100635,
http://linkedlifedata.com/resource/pubmed/chemical/dolaisoleucine,
http://linkedlifedata.com/resource/pubmed/chemical/gamma-Aminobutyric Acid
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
0304-3940
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
11
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| pubmed:volume |
439
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
138-42
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| pubmed:meshHeading |
pubmed-meshheading:18524490-8-Hydroxy-2-(di-n-propylamino)tetralin,
pubmed-meshheading:18524490-Amino Acids,
pubmed-meshheading:18524490-Animals,
pubmed-meshheading:18524490-Drug Interactions,
pubmed-meshheading:18524490-Male,
pubmed-meshheading:18524490-Membrane Potentials,
pubmed-meshheading:18524490-Neural Inhibition,
pubmed-meshheading:18524490-Neurons,
pubmed-meshheading:18524490-Paraventricular Hypothalamic Nucleus,
pubmed-meshheading:18524490-Patch-Clamp Techniques,
pubmed-meshheading:18524490-Piperazines,
pubmed-meshheading:18524490-Piperidones,
pubmed-meshheading:18524490-Pyridines,
pubmed-meshheading:18524490-Rats,
pubmed-meshheading:18524490-Rats, Sprague-Dawley,
pubmed-meshheading:18524490-Serotonin,
pubmed-meshheading:18524490-Serotonin Antagonists,
pubmed-meshheading:18524490-Spiro Compounds,
pubmed-meshheading:18524490-Synaptic Transmission,
pubmed-meshheading:18524490-gamma-Aminobutyric Acid
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| pubmed:year |
2008
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| pubmed:articleTitle |
Serotonin inhibits GABA synaptic transmission in presympathetic paraventricular nucleus neurons.
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| pubmed:affiliation |
Research Institute of Medical Sciences, Department of Physiology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Joong-gu, Daejeon, 301-131, Republic of Korea.
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| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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