Linked Life Data

18523922

Source:http://linkedlifedata.com/resource/pubmed/id/18523922

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2008-7-7
pubmed:abstractText
A rapid and simple high-performance liquid chromatographic (HPLC) method was developed and validated to simultaneously analyze the diastereomers of (+)-licarin A and isolicarin A in rat plasma after intravenous administration. The analytes were extracted from the plasma by solid-phase extraction (SPE). Diastereomeric separation and determination were successfully achieved using a Diamonsil ODS C (18) reversed-phase column (250 mm x 4.6 mm i. d., 5 microm) with an RP18 guard column (8 mm x 4.6 mm i. d., 5 microm) and a mobile phase of MeOH-H (2)O (4 : 1, v/v). UV detection was at 270 nm. The linear ranges of the standard curves were 0.25 - 150.00 microg/mL for (+)-licarin A and 0.10 - 75.00 microg/mL for isolicarin A. The lower limits of detection and quantification were 0.05 and 0.25 microg/mL for (+)-licarin A, and 0.05 and 0.10 microg/mL for isolicarin A, respectively. This assay method was successfully applied to study the pharmacokinetics of diastereomers (+)-licarin A and isolicarin A in rat plasma.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0032-0943
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
880-4
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Simultaneous determination of diastereomers (+)-licarin A and isolicarin A from Myristica fragrans in rat plasma by HPLC and its application to their pharmacokinetics.
pubmed:affiliation
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Validation Studies