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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2008-6-4
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pubmed:abstractText |
Separate populations of M cells have been detected in the follicle-associated epithelium of Peyer's patches (PPs) and the villous epithelium of the small intestine, but the traits shared by or distinguishing the two populations have not been characterized. Our separate study has demonstrated that a potent mucosal modulator cholera toxin (CT) can induce lectin Ulex europaeus agglutinin-1 and our newly developed M cell-specific mAb NKM 16-2-4-positive M-like cells in the duodenal villous epithelium. In this study, we determined the gene expression of PP M cells, CT-induced villous M-like cells, and intestinal epithelial cells isolated by a novel approach using FACS. Additional mRNA and protein analyses confirmed the specific expression of glycoprotein 2 and myristoylated alanine-rich C kinase substrate (MARCKS)-like protein by PP M cells but not CT-induced villous M-like cells. Comprehensive gene profiling also suggested that CT-induced villous M-like cells share traits of both PP M cells and intestinal epithelial cells, a finding that is supported by their unique expression of specific chemokines. The genome-wide assessment of gene expression facilitates discovery of M cell-specific molecules and enhances the molecular understanding of M cell immunobiology.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1767
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pubmed:author |
pubmed-author:HaseKojiK,
pubmed-author:IgarashiOsamuO,
pubmed-author:KiyonoHiroshiH,
pubmed-author:KurokawaShihoS,
pubmed-author:LoweAnson WAW,
pubmed-author:MejimaMioM,
pubmed-author:NochiTomonoriT,
pubmed-author:OhnoHiroshiH,
pubmed-author:PontesGemilson SoaresGS,
pubmed-author:TakayamaNaokoN,
pubmed-author:TeraharaKazutakaK,
pubmed-author:YoshidaMasatoM,
pubmed-author:YukiYoshikazuY
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pubmed:issnType |
Print
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pubmed:day |
15
|
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pubmed:volume |
180
|
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pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
7840-6
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:18523247-Animals,
pubmed-meshheading:18523247-Cell Line,
pubmed-meshheading:18523247-Chemokines,
pubmed-meshheading:18523247-Cholera Toxin,
pubmed-meshheading:18523247-Duodenum,
pubmed-meshheading:18523247-Flow Cytometry,
pubmed-meshheading:18523247-GPI-Linked Proteins,
pubmed-meshheading:18523247-Gene Expression Profiling,
pubmed-meshheading:18523247-Immunohistochemistry,
pubmed-meshheading:18523247-Intestinal Mucosa,
pubmed-meshheading:18523247-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:18523247-Membrane Glycoproteins,
pubmed-meshheading:18523247-Mice,
pubmed-meshheading:18523247-Mice, Inbred BALB C,
pubmed-meshheading:18523247-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:18523247-Peyer's Patches,
pubmed-meshheading:18523247-Rats
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pubmed:year |
2008
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pubmed:articleTitle |
Comprehensive gene expression profiling of Peyer's patch M cells, villous M-like cells, and intestinal epithelial cells.
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pubmed:affiliation |
Division of Mucosal Immunology, Graduate School of Frontier Science, The University of Tokyo, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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