Source:http://linkedlifedata.com/resource/pubmed/id/18521533
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2008-7-31
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pubmed:abstractText |
We have investigated the ability of satellite DNA beta to complement mutations in the CP, V2 and C4 genes of the monopartite begomovirus, tomato leaf curl virus, which are potentially involved in movement. A mutation in the coat protein was not complemented by DNA beta. Mutations of the C4 and V2 genes attenuated and abolished symptoms, respectively. In the presence of the C4 mutant, but not the V2 mutant, DNA beta induced typical symptoms, confirming that the satellite encodes a dominant symptom determinant. In contrast to the C4 mutant, DNA beta did not enhance the viral DNA levels of the V2 mutant, suggesting that V2 is required for this phenomenon. The significance of these findings is discussed based on our present understanding of the functions of the viral genes and DNA beta.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0304-8608
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
153
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1367-72
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pubmed:meshHeading |
pubmed-meshheading:18521533-Begomovirus,
pubmed-meshheading:18521533-Blotting, Southern,
pubmed-meshheading:18521533-Capsid Proteins,
pubmed-meshheading:18521533-DNA, Satellite,
pubmed-meshheading:18521533-Gene Deletion,
pubmed-meshheading:18521533-Genetic Complementation Test,
pubmed-meshheading:18521533-Phenotype,
pubmed-meshheading:18521533-Plant Diseases,
pubmed-meshheading:18521533-Tobacco
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pubmed:year |
2008
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pubmed:articleTitle |
Satellite DNA beta overrides the pathogenicity phenotype of the C4 gene of tomato leaf curl virus but does not compensate for loss of function of the coat protein and V2 genes.
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pubmed:affiliation |
National Institute for Biotechnology and Genetic Engineering, PO Box 577, Jhang Road, Faisalabad, Pakista., msaeed@nibge.org
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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