Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2008-6-3
pubmed:abstractText
The occurrence and distribution of (1,4)-beta-galactan in wood cells of poplar (Populus trichocarpa Torr. & A. Gray x P. koreana Rehder) were studied by immunolabeling with a monoclonal antibody (LM5) specific to this carbohydrate epitope. Immunofluorescence microscopy revealed exclusive binding of the LM5 antibody to tension wood fibers, indicating the specific presence of (1,4)-beta-galactan in cell walls of this wood cell type. Higher magnifications achieved with the fluorescence microscope and additional immunogold electron microscopy showed that the binding of the LM5 antibody was mainly restricted to a narrow cell wall area between the gelatinous G-layer and the secondary cell wall. This labeling pattern strongly suggests a role of (1,4)-beta-galactan in cross-linking the G-layer and secondary cell wall. Furthermore, the exclusive localization of (1,4)-beta-galactan in tension wood strengthens the view that this carbohydrate epitope can be considered a highly specific marker of reaction wood formation in mechanically stressed trees.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0829-318X
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1263-7
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Immunolocalization of (1,4)-beta-galactan in tension wood fibers of poplar.
pubmed:affiliation
Technische Universität München, Fachgebiet Angewandte Holzbiologie, Winzererstrasse 45, München, Germany. arend@wzw.tum.de
pubmed:publicationType
Journal Article, Evaluation Studies