Source:http://linkedlifedata.com/resource/pubmed/id/18516617
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2008-6-19
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| pubmed:abstractText |
The role of nitric oxide (NO)- and prostacyclin (PGI(2))-independent mechanism, potentially attributable to endothelium-derived hyperpolarizing factor (EDHF), has not been extensively studied in human skin microcirculation. The aim of our study was to elucidate the contribution of the NO- and PGI(2)-independent mechanism to microvascular reactivity of cutaneous microcirculation. Skin perfusion was measured on the volar aspect of the forearm in 12 healthy male subjects (mean age 25.0 +/- 1.5), using laser Doppler (LD) fluxmetry. Combined endothelial nitric oxide synthase (eNOS) and cyclooxygenase (COX) inhibition was achieved by an intradermal injection (10 microl) of the eNOS inhibitor, L(omega)-monomethyl L-arginine (L-NMMA, 10 mM) and the COX inhibitor, diclofenac (10 mM); saline was injected as a control. LD flux was assessed at rest and after an iontophoretical application of acetylcholine (ACh, 1%), an endothelial agonist and sodium nitroprusside (SNP, 1%), an endothelium-independent agonist, respectively. Combined eNOS and COX inhibition had no effect on the baseline LDF (12.5 +/- 2.3 PU (perfusion units) in control vs. 10.9 +/- 1.8 PU in the treated site). On the other hand, the ACh-stimulated increase in LDF was significantly attenuated after eNOS and COX inhibition (390.5 +/- 43.5%), compared to the control (643.7 +/- 80.3% increase, t-test, P < 0.05). Nevertheless, at least 60% of ACh-mediated vasodilatation was preserved after combined eNOS and COX inhibition. eNOS and COX inhibition had no impact on the SNP-stimulated increase in LDF (768.8 +/- 70.5% in control vs. 733.5 +/- 54.6% in the treated site). These findings indicate that NO- and PGI(2)-independent mechanism plays an important role in the regulation of blood flow in the human skin microcirculation.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Diclofenac,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Epoprostenol,
http://linkedlifedata.com/resource/pubmed/chemical/NOS3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type III,
http://linkedlifedata.com/resource/pubmed/chemical/Nitroprusside,
http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents,
http://linkedlifedata.com/resource/pubmed/chemical/endothelium-dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/omega-N-Methylarginine
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
1439-6319
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
103
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
719-26
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| pubmed:meshHeading |
pubmed-meshheading:18516617-Acetylcholine,
pubmed-meshheading:18516617-Adult,
pubmed-meshheading:18516617-Biological Factors,
pubmed-meshheading:18516617-Blood Flow Velocity,
pubmed-meshheading:18516617-Cyclooxygenase Inhibitors,
pubmed-meshheading:18516617-Diclofenac,
pubmed-meshheading:18516617-Enzyme Inhibitors,
pubmed-meshheading:18516617-Epoprostenol,
pubmed-meshheading:18516617-Forearm,
pubmed-meshheading:18516617-Humans,
pubmed-meshheading:18516617-Injections, Intradermal,
pubmed-meshheading:18516617-Iontophoresis,
pubmed-meshheading:18516617-Laser-Doppler Flowmetry,
pubmed-meshheading:18516617-Male,
pubmed-meshheading:18516617-Microcirculation,
pubmed-meshheading:18516617-Nitric Oxide,
pubmed-meshheading:18516617-Nitric Oxide Synthase Type III,
pubmed-meshheading:18516617-Nitroprusside,
pubmed-meshheading:18516617-Regional Blood Flow,
pubmed-meshheading:18516617-Skin,
pubmed-meshheading:18516617-Time Factors,
pubmed-meshheading:18516617-Vasodilation,
pubmed-meshheading:18516617-Vasodilator Agents,
pubmed-meshheading:18516617-omega-N-Methylarginine
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| pubmed:year |
2008
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| pubmed:articleTitle |
The effect of nitric oxide synthase and cyclooxygenase inhibition on cutaneous microvascular reactivity.
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| pubmed:affiliation |
Institute of Physiology, School of Medicine, University of Ljubljana, Zaloska 4, Ljubljana, Slovenia. helena.lenasi.ml@mf.uni-lj.si
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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