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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
2008-7-15
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pubmed:abstractText |
Time-of-flight mass spectrometry (TOF MS) is increasingly used in proteomics research. Herein, we report on the development and characterization of a TOF MS instrument with improved sensitivity equipped with an electrodynamic ion funnel trap (IFT) that employs an automated gain control (AGC) capability. The IFT-TOF MS was coupled to a reversed-phase capillary liquid chromatography (RPLC) separation and evaluated in experiments with complex proteolytic digests. When applied to a global tryptic digest of Shewanella oneidensis proteins, an order-of-magnitude increase in sensitivity compared to that of the conventional continuous mode of operation was achieved due to efficient ion accumulation prior to TOF MS analysis. As a result of this sensitivity improvement and related improvement in mass measurement accuracy, the number of unique peptides identified in the AGC-IFT mode was 5-fold greater than that obtained in the continuous mode.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-10631658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-10795088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-11013419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-11473401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-11569805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-12717759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-14632135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-15694774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-15889897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-16429408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-16808456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-16926098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-17472340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-17545182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-17850113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-18044960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-2134340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-9364793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-9784749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18512944-9949732
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1520-6882
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5367-76
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pubmed:dateRevised |
2011-1-25
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Automated gain control ion funnel trap for orthogonal time-of-flight mass spectrometry.
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pubmed:affiliation |
Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural
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