Linked Life Data

18511208

Source:http://linkedlifedata.com/resource/pubmed/id/18511208

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-6-18
pubmed:abstractText
Circadian behavioral rhythms in mammals are controlled by a central clock located in the suprachiasmatic nucleus (SCN). PER2, the protein product of the clock gene, Period 2 (Per2), is expressed rhythmically in the SCN [Beaule C, Houle LM, Amir S (2003) Expression profiles of PER2 immunoreactivity within the shell and core regions of the rat suprachiasmatic nucleus: Lack of effect of photic entrainment and disruption by constant light. J Mol Neurosci 21:133-148] and has been implicated in the control of circadian behavioral rhythms based on the evidence that genetic mutations in Per2 abolish free running locomotor activity rhythms in mice [Zheng B, Larkin DW, Albrecht U, Sun ZS, Sage M, Eichele G, Lee CC, Bradley A (1999) The mPer2 gene encodes a functional component of the mammalian circadian clock. Nature 400:169-173; Bae K, Jin X, Maywood ES, Hastings MH, Reppert SM, Weaver DR (2001) Differential functions of mPer1, mPer2, and mPer3 in the SCN circadian clock. Neuron 30:525-536]. Such mutations eradicate PER2 expression in the SCN and disrupt the SCN molecular clockwork, however, they also affect PER2 in the rest of the brain and body leaving open the possibility that the changes in behavioral rhythms might be influenced, at least in part, by disruptions in PER2 functioning outside the SCN. We used RNA interference-mediated transient knockdown of Per2 to study the effect of selective suppression of PER2 expression in the SCN, per se, on behavioral circadian rhythms. We found that transient suppression of PER2 in the SCN disrupted free running locomotor activity rhythms for up to 10 days in rats. Infusions of control dsRNA into the SCN or infusions of dsRNA to Per2 immediately dorsal to the SCN had no effect. These results constitute evidence for a direct link between PER2 expression in the SCN and the expression of behavioral circadian rhythms in mammals.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0306-4522
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
154
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
409-14
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:18511208-Animals, pubmed-meshheading:18511208-Cell Cycle Proteins, pubmed-meshheading:18511208-Circadian Rhythm, pubmed-meshheading:18511208-Data Interpretation, Statistical, pubmed-meshheading:18511208-Gene Expression, pubmed-meshheading:18511208-Genes, fos, pubmed-meshheading:18511208-Globins, pubmed-meshheading:18511208-Immunohistochemistry, pubmed-meshheading:18511208-Limbic System, pubmed-meshheading:18511208-Male, pubmed-meshheading:18511208-Microinjections, pubmed-meshheading:18511208-Motor Activity, pubmed-meshheading:18511208-Nuclear Proteins, pubmed-meshheading:18511208-Period Circadian Proteins, pubmed-meshheading:18511208-Prosencephalon, pubmed-meshheading:18511208-RNA, Double-Stranded, pubmed-meshheading:18511208-RNA Interference, pubmed-meshheading:18511208-Rats, pubmed-meshheading:18511208-Rats, Wistar, pubmed-meshheading:18511208-Suprachiasmatic Nucleus
pubmed:year
2008
pubmed:articleTitle
Double-stranded RNA-mediated suppression of Period2 expression in the suprachiasmatic nucleus disrupts circadian locomotor activity in rats.
pubmed:affiliation
Center for Studies in Behavioral Neurobiology, Department of Psychology, Concordia University, SP-244, Montréal, QC, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural