Linked Life Data

1850919

Source:http://linkedlifedata.com/resource/pubmed/id/1850919

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-6-6
pubmed:databankReference
pubmed:abstractText
cDNA libraries from porcine group (Gp) C rotavirus strain Cowden and a human Gp C rotavirus strain were generated. The complete nucleotide sequence of gene 8 from the Cowden strain was determined from gene 8-specific clones and viral transcript RNA. A full-length gene 8 clone was generated from the human Gp C virus by polymerase chain reaction (PCR) using primers deduced from the 3' and 5' ends of the Cowden strain gene 8, and the sequence of the human Gp C gene 8 was determined from this clone and gene 8 clones in the cDNA library. The gene 8 from the Cowden or the human Gp C strain is 1063 nucleotides in length and contains a long open reading frame beginning at the 49th nucleotide from the 5' end and terminating with a stop codon 16 bases from the 3' end. The encoded protein contains 332 amino acids (predicted molecular weight of 37.3 kDa) with two potential N-linked glycosylation sites in the porcine strain and three in the human strain. The polypeptide products derived from in vitro translation of the transcript RNA generated from a porcine gene 8 clone containing the entire open reading frame were analogous in size with the Gp A VP7. The gene 8 of porcine and human Gp C rotaviruses exhibited considerable nucleotide and deduced amino acid sequence identity (83.8 and 88.0%, respectively). Comparison of the Gp C gene 8 protein sequence with the VP7 protein of Gp A rotavirus revealed structural similarities, although the overall amino acid identity was low (less than 30%). These data suggest that the gene 8 of the porcine or human Gp C rotavirus encodes a protein corresponding to the VP7 outer capsid glycoprotein of Gp A rotaviruses and that the eighth gene is highly conserved in the porcine and human Gp C strains examined in this study.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
182
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
562-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Sequence conservation of gene 8 between human and porcine group C rotaviruses and its relationship to the VP7 gene of group A rotaviruses.
pubmed:affiliation
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro