Linked Life Data

18508999

Source:http://linkedlifedata.com/resource/pubmed/id/18508999

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-5-29
pubmed:abstractText
Adrenal tumors occur more frequently in women and are the leading cause of Cushing's syndrome during pregnancy. We aimed to evaluate the potential role of sex steroids in the susceptibility of women to adrenocortical tumors. We evaluated the presence of the progesterone receptor (PR), estradiol receptors (ERs), and aromatase in 5 patients with primary pigmented nodular adrenal disease (PPNAD), 15 adrenocortical adenomas (ACAs) and adjacent normal tissues, 12 adrenocortical carcinomas (ACCs), and 3 normal adrenal glands (NA). The expression of PR and ERalpha was evaluated by enzyme immunoassays, real-time RT-PCR, immunohistochemistry, and cytosol-based ligand-binding assays. ERbeta and aromatase levels were evaluated by real-time RT-PCR. ERalpha concentrations were low in NA, in adrenal tissues adjacent to ACA (51+/-33), in ACC (53+/-78), and lower in ACA (11+/-11 fmol/mg DNA). Conversely, PR concentrations were high in NA and adrenal tissues adjacent to ACA, at 307+/-216 fmol/mg DNA, and were even higher in tumors - 726+/-706 fmol/mg DNA in ACA and 1154+/-1586 fmol/mg DNA in ACC - and in isolated PPNAD nodules. Binding study results in four tumors were compatible with binding to a steroid receptor. In patients with PPNAD, a strong positive immunohistochemical signal was associated with the sole isolated nodular regions. ERbeta transcript levels were very high in all samples except those for two ACCs, whereas aromatase levels were low. PR and ERbeta are clearly present in normal adrenal glands and adrenal tumors. Further studies may shed light on the possible pathogenic role of these receptors in adrenal proliferation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1351-0088
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
465-74
pubmed:meshHeading
pubmed-meshheading:18508999-Adolescent, pubmed-meshheading:18508999-Adrenal Cortex, pubmed-meshheading:18508999-Adrenal Cortex Diseases, pubmed-meshheading:18508999-Adrenal Cortex Neoplasms, pubmed-meshheading:18508999-Adrenocortical Adenoma, pubmed-meshheading:18508999-Adult, pubmed-meshheading:18508999-Aged, pubmed-meshheading:18508999-Aged, 80 and over, pubmed-meshheading:18508999-Aromatase, pubmed-meshheading:18508999-Child, pubmed-meshheading:18508999-Cytosol, pubmed-meshheading:18508999-Estrogen Receptor alpha, pubmed-meshheading:18508999-Estrogen Receptor beta, pubmed-meshheading:18508999-Female, pubmed-meshheading:18508999-Gene Expression Regulation, Neoplastic, pubmed-meshheading:18508999-Humans, pubmed-meshheading:18508999-Male, pubmed-meshheading:18508999-Middle Aged, pubmed-meshheading:18508999-RNA, Messenger, pubmed-meshheading:18508999-Receptors, Progesterone
pubmed:year
2008
pubmed:articleTitle
Expression of progesterone and estradiol receptors in normal adrenal cortex, adrenocortical tumors, and primary pigmented nodular adrenocortical disease.
pubmed:affiliation
Unité de Pharmacologie, Institut Curie, 26 rue d'Ulm, 75005 Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't