| pubmed-article:18506428 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18506428 | lifeskim:mentions | umls-concept:C1095831 | lld:lifeskim |
| pubmed-article:18506428 | lifeskim:mentions | umls-concept:C0004755 | lld:lifeskim |
| pubmed-article:18506428 | lifeskim:mentions | umls-concept:C0002520 | lld:lifeskim |
| pubmed-article:18506428 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:18506428 | lifeskim:mentions | umls-concept:C2828366 | lld:lifeskim |
| pubmed-article:18506428 | lifeskim:mentions | umls-concept:C1705851 | lld:lifeskim |
| pubmed-article:18506428 | lifeskim:mentions | umls-concept:C1555707 | lld:lifeskim |
| pubmed-article:18506428 | lifeskim:mentions | umls-concept:C2752151 | lld:lifeskim |
| pubmed-article:18506428 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:18506428 | pubmed:dateCreated | 2008-7-30 | lld:pubmed |
| pubmed-article:18506428 | pubmed:abstractText | A method has been developed for quantification of 20 amino acids as well as 13 (15)N-labeled amino acids in barley plants. The amino acids were extracted from plant tissues using aqueous HCl-ethanol and directly analyzed without further purification. Analysis of the underivatized amino acids was performed by liquid chromatography (LC)-electrospray ionization (ESI) tandem mass spectrometry (MS-MS) in the positive ESI mode. Separation was achieved on a strong cation exchange column (Luna 5micro SCX 100A) with 30 mM ammonium acetate in water (solvent A) and 5% acetic acid in water (solvent B). Quantification was accomplished using d (2)-Phe as an internal standard. Calibration curves were linear over the range 0.5-50 microM, and limits of detection were estimated to be 0.1-3.0 microM. The mass-spectrometric technique was employed to study the regulation of amino acid levels in barley plants grown at 15 degrees C uniform root temperature (RT) and 20-10 degrees C vertical RT gradient (RTG). The LC-MS-MS results demonstrated enhanced concentration of free amino acids in shoots at 20-10 degrees C RTG, while total free amino acid concentration in roots was similarly low for both RT treatments. (15)NO(3) (-) labeling experiments showed lower (15)N/(14)N ratios for Glu, Ser, Ala and Val in plants grown at 20-10 degrees C RTG compared with those grown at 15 degrees C RT. | lld:pubmed |
| pubmed-article:18506428 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18506428 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18506428 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18506428 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18506428 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18506428 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18506428 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18506428 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18506428 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:18506428 | pubmed:issn | 1618-2650 | lld:pubmed |
| pubmed-article:18506428 | pubmed:author | pubmed-author:KuhnArnd JAJ | lld:pubmed |
| pubmed-article:18506428 | pubmed:author | pubmed-author:FüllnerKersti... | lld:pubmed |
| pubmed-article:18506428 | pubmed:author | pubmed-author:HofmannDianaD | lld:pubmed |
| pubmed-article:18506428 | pubmed:author | pubmed-author:OldigesMarcoM | lld:pubmed |
| pubmed-article:18506428 | pubmed:author | pubmed-author:ThieleBjörnB | lld:pubmed |
| pubmed-article:18506428 | pubmed:author | pubmed-author:SteinNadineN | lld:pubmed |
| pubmed-article:18506428 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18506428 | pubmed:volume | 391 | lld:pubmed |
| pubmed-article:18506428 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18506428 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18506428 | pubmed:pagination | 2663-72 | lld:pubmed |
| pubmed-article:18506428 | pubmed:meshHeading | pubmed-meshheading:18506428... | lld:pubmed |
| pubmed-article:18506428 | pubmed:meshHeading | pubmed-meshheading:18506428... | lld:pubmed |
| pubmed-article:18506428 | pubmed:meshHeading | pubmed-meshheading:18506428... | lld:pubmed |
| pubmed-article:18506428 | pubmed:meshHeading | pubmed-meshheading:18506428... | lld:pubmed |
| pubmed-article:18506428 | pubmed:meshHeading | pubmed-meshheading:18506428... | lld:pubmed |
| pubmed-article:18506428 | pubmed:meshHeading | pubmed-meshheading:18506428... | lld:pubmed |
| pubmed-article:18506428 | pubmed:meshHeading | pubmed-meshheading:18506428... | lld:pubmed |
| pubmed-article:18506428 | pubmed:meshHeading | pubmed-meshheading:18506428... | lld:pubmed |
| pubmed-article:18506428 | pubmed:meshHeading | pubmed-meshheading:18506428... | lld:pubmed |
| pubmed-article:18506428 | pubmed:meshHeading | pubmed-meshheading:18506428... | lld:pubmed |
| pubmed-article:18506428 | pubmed:meshHeading | pubmed-meshheading:18506428... | lld:pubmed |
| pubmed-article:18506428 | pubmed:meshHeading | pubmed-meshheading:18506428... | lld:pubmed |
| pubmed-article:18506428 | pubmed:meshHeading | pubmed-meshheading:18506428... | lld:pubmed |
| pubmed-article:18506428 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18506428 | pubmed:articleTitle | Analysis of amino acids without derivatization in barley extracts by LC-MS-MS. | lld:pubmed |
| pubmed-article:18506428 | pubmed:affiliation | Institute for Chemistry and Dynamics of the Geosphere, Institute-3: Phytosphere/BioSpec, Forschungszentrum Jülich GmbH, 52425, Juelich, Germany. b.thiele@fz-juelich.de | lld:pubmed |
| pubmed-article:18506428 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18506428 | lld:pubmed |
