Linked Life Data

1850419

Source:http://linkedlifedata.com/resource/pubmed/id/1850419

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1991-5-29
pubmed:abstractText
To study the mechanisms which mediate the transcriptional activation of cardiac genes during alpha adrenergic stimulation, the present study examined the regulated expression of three cardiac genes, a ventricular embryonic gene (atrial natriuretic factor, ANF), a constitutively expressed contractile protein gene (cardiac MLC-2), and a cardiac sodium channel gene. alpha 1-Adrenergic stimulation activates the expression and release of ANF from neonatal ventricular cells. As assessed by RNase protection analyses, treatment with alpha-adrenergic agonists increases the steady-state levels of ANF mRNA by greater than 15-fold. However, a rat cardiac sodium channel gene mRNA is not induced, indicating that alpha-adrenergic stimulation does not lead to an increase in the expression of all cardiac genes. Studies employing a series of rat ANF luciferase and rat MLC-2 luciferase fusion genes identify 315- and 92-base pair cis regulatory sequences within an embryonic gene (ANF) and a constitutively expressed contractile protein gene (MLC-2), respectively, which mediate alpha-adrenergic-inducible gene expression. Transfection of various ANF luciferase reporters into neonatal rat ventricular cells demonstrated that upstream sequences which mediate tissue-specific expression (-3003 to -638) can be segregated from those responsible for inducibility. The lack of inducibility of a cardiac Na+ channel gene, and the segregation of ANF gene sequences which mediate cardiac specific from those which mediate inducible expression, provides further insight into the relationship between muscle-specific and inducible expression during cardiac myocyte hypertrophy. Based on these results, a testable model is proposed for the induction of embryonic cardiac genes and constitutively expressed contractile protein genes and the noninducibility of a subset of cardiac genes during alpha-adrenergic stimulation of neonatal rat ventricular cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
266
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7759-68
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:1850419-Animals, pubmed-meshheading:1850419-Atrial Natriuretic Factor, pubmed-meshheading:1850419-Base Sequence, pubmed-meshheading:1850419-Chimera, pubmed-meshheading:1850419-Embryo, Mammalian, pubmed-meshheading:1850419-Fluorescent Antibody Technique, pubmed-meshheading:1850419-Gene Expression Regulation, pubmed-meshheading:1850419-Heart Ventricles, pubmed-meshheading:1850419-Luciferases, pubmed-meshheading:1850419-Molecular Sequence Data, pubmed-meshheading:1850419-Myocardium, pubmed-meshheading:1850419-Myosins, pubmed-meshheading:1850419-Norepinephrine, pubmed-meshheading:1850419-RNA, Messenger, pubmed-meshheading:1850419-Rats, pubmed-meshheading:1850419-Rats, Inbred Strains, pubmed-meshheading:1850419-Receptors, Adrenergic, alpha, pubmed-meshheading:1850419-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:1850419-Sodium Channels, pubmed-meshheading:1850419-Transfection
pubmed:year
1991
pubmed:articleTitle
Co-regulation of the atrial natriuretic factor and cardiac myosin light chain-2 genes during alpha-adrenergic stimulation of neonatal rat ventricular cells. Identification of cis sequences within an embryonic and a constitutive contractile protein gene which mediate inducible expression.
pubmed:affiliation
Department of Medicine, University of California, San Diego, La Jolla 92093.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't