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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1991-5-24
|
| pubmed:abstractText |
In amphibian and mammalian systems, regulation of Na+ transport via the Na,K-ATPase plays an important role in distinct developmental processes such as blastocoele formation and neurulation. In this study, we have followed the Na,K-ATPase activity, the biosynthesis, and the cellular accumulation of catalytic alpha-subunits after fertilization of Xenopus laevis eggs up to neurula formation. Our data show that Na,K-ATPase activity increases significantly between stages 4 and 6 and again between stages 13 and 24. The four-fold rise in Na,K-ATPase activity during blastocoele formation is not mediated by an increased cellular pool of alpha-subunits. On the other hand, a five-fold increase of the biosynthesis rate around midblastula precedes a progressive accumulation up to neurula stage mainly of alpha 1-subunits and to a lesser extent of a second alpha-immunoreactive species. In contrast, newly synthesized glycoproteinic beta 1-subunits of Na,K-ATPase cannot be detected up to late neurula. These data indicate that (1) upregulation of Na,K-ATPase activity during blastocoele and neurula formation are mediated by different regulation mechanisms and (2) alpha- and possibly beta-isoforms are expressed in a developmentally regulated fashion during early Xenopus development.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0012-1606
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
145
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
174-81
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1850368-Animals,
pubmed-meshheading:1850368-Blotting, Western,
pubmed-meshheading:1850368-Ca(2+) Mg(2+)-ATPase,
pubmed-meshheading:1850368-Embryo, Nonmammalian,
pubmed-meshheading:1850368-Female,
pubmed-meshheading:1850368-Kinetics,
pubmed-meshheading:1850368-Macromolecular Substances,
pubmed-meshheading:1850368-Male,
pubmed-meshheading:1850368-Microsomes,
pubmed-meshheading:1850368-Sodium-Potassium-Exchanging ATPase,
pubmed-meshheading:1850368-Xenopus laevis
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Modulation of Na,K-ATPase expression during early development of Xenopus laevis.
|
| pubmed:affiliation |
Institut de Pharmacologie et de Toxicologie de l'Université, Lausanne, Switzerland.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|