| pubmed-article:1850168 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1850168 | lifeskim:mentions | umls-concept:C0206419 | lld:lifeskim |
| pubmed-article:1850168 | lifeskim:mentions | umls-concept:C0005767 | lld:lifeskim |
| pubmed-article:1850168 | lifeskim:mentions | umls-concept:C0806987 | lld:lifeskim |
| pubmed-article:1850168 | lifeskim:mentions | umls-concept:C0017982 | lld:lifeskim |
| pubmed-article:1850168 | lifeskim:mentions | umls-concept:C1610033 | lld:lifeskim |
| pubmed-article:1850168 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:1850168 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:1850168 | lifeskim:mentions | umls-concept:C1546857 | lld:lifeskim |
| pubmed-article:1850168 | lifeskim:mentions | umls-concept:C1556066 | lld:lifeskim |
| pubmed-article:1850168 | lifeskim:mentions | umls-concept:C1619636 | lld:lifeskim |
| pubmed-article:1850168 | lifeskim:mentions | umls-concept:C1514873 | lld:lifeskim |
| pubmed-article:1850168 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:1850168 | pubmed:dateCreated | 1991-5-17 | lld:pubmed |
| pubmed-article:1850168 | pubmed:abstractText | Porcine peripheral blood mononuclear cells (PBMC) are induced to produce interferon alpha (IFN alpha) following in vitro exposure to coronavirus TGEV (transmissible gastroenteritis virus)-infected glutaraldehyde-fixed cell monolayers or to TGEV virions. In the present report, we examined the possibility that glycosylation of viral proteins could play a major role in interactions with PBMC leading to the production of IFN alpha. Con A pretreatment of TGEV-infected cell monolayers before fixation with glutaraldehyde and exposure to PBMC caused a dose-dependent inhibition of IFN alpha induction, implying that masking of carbohydrates at the surface of infected cells lowered IFN-alpha-induction. Similarly, inhibition of N-linked glycosylation by tunicamycin during viral infection of cell monolayers altered their ability to induce IFN alpha. In addition, complete cleavage of 'complex type' oligosaccharides by peptide-N-glycohydrolase F lowered the capacity of TGEV virions to induce IFN alpha. Thus, these findings strongly suggest that glycosylation of the viral proteins, and more precisely the presence of complex-type oligosaccharides, is an important requirement for a completely efficient interaction with PBMC leading to the production of IFN-alpha. | lld:pubmed |
| pubmed-article:1850168 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1850168 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850168 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1850168 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850168 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850168 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850168 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850168 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850168 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1850168 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1850168 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:1850168 | pubmed:issn | 0300-9475 | lld:pubmed |
| pubmed-article:1850168 | pubmed:author | pubmed-author:CharletMM | lld:pubmed |
| pubmed-article:1850168 | pubmed:author | pubmed-author:LavenantLL | lld:pubmed |
| pubmed-article:1850168 | pubmed:author | pubmed-author:DelmasBB | lld:pubmed |
| pubmed-article:1850168 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1850168 | pubmed:volume | 33 | lld:pubmed |
| pubmed-article:1850168 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1850168 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1850168 | pubmed:pagination | 435-40 | lld:pubmed |
| pubmed-article:1850168 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:meshHeading | pubmed-meshheading:1850168-... | lld:pubmed |
| pubmed-article:1850168 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1850168 | pubmed:articleTitle | Glycosylation is required for coronavirus TGEV to induce an efficient production of IFN alpha by blood mononuclear cells. | lld:pubmed |
| pubmed-article:1850168 | pubmed:affiliation | Laboratoire de Virologie et d'Immunologie Moléculaires, I.N.R.A., Centre de Recherches de Jouy-en-Josas, France. | lld:pubmed |
| pubmed-article:1850168 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1850168 | pubmed:publicationType | In Vitro | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850168 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850168 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850168 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850168 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850168 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850168 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1850168 | lld:pubmed |
