Linked Life Data

1850168

Source:http://linkedlifedata.com/resource/pubmed/id/1850168

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1991-5-17
pubmed:abstractText
Porcine peripheral blood mononuclear cells (PBMC) are induced to produce interferon alpha (IFN alpha) following in vitro exposure to coronavirus TGEV (transmissible gastroenteritis virus)-infected glutaraldehyde-fixed cell monolayers or to TGEV virions. In the present report, we examined the possibility that glycosylation of viral proteins could play a major role in interactions with PBMC leading to the production of IFN alpha. Con A pretreatment of TGEV-infected cell monolayers before fixation with glutaraldehyde and exposure to PBMC caused a dose-dependent inhibition of IFN alpha induction, implying that masking of carbohydrates at the surface of infected cells lowered IFN-alpha-induction. Similarly, inhibition of N-linked glycosylation by tunicamycin during viral infection of cell monolayers altered their ability to induce IFN alpha. In addition, complete cleavage of 'complex type' oligosaccharides by peptide-N-glycohydrolase F lowered the capacity of TGEV virions to induce IFN alpha. Thus, these findings strongly suggest that glycosylation of the viral proteins, and more precisely the presence of complex-type oligosaccharides, is an important requirement for a completely efficient interaction with PBMC leading to the production of IFN-alpha.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0300-9475
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
435-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Glycosylation is required for coronavirus TGEV to induce an efficient production of IFN alpha by blood mononuclear cells.
pubmed:affiliation
Laboratoire de Virologie et d'Immunologie Moléculaires, I.N.R.A., Centre de Recherches de Jouy-en-Josas, France.
pubmed:publicationType
Journal Article, In Vitro