Source:http://linkedlifedata.com/resource/pubmed/id/18500584
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2008-5-26
|
| pubmed:abstractText |
Two xylanases were isolated and purified from crude culture filtrate of Aspergillus sydowii SBS 45 after 9 days of growth on wheat bran containing 0.5% (w/v) birch wood xylan as the carbon source under solid-state fermentation. After a three-step purification scheme involving ammonium sulfate precipitation, gel filtration chromatography (Sephadex G-200), and anion exchange chromatography (DEAE-Sephadex A-50), xylanase I was purified 93.41 times, and xylanase II was purified 77.40 times with yields of 4.49 and 10.46, respectively. Molecular weights of xylanase I and II were 20.1 and 43 kDa, respectively, in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Optimum temperature was 50 degrees C, and optimum pH was 10.0 for both xylanase I and II. The Km value of xylanase I for birch wood xylan was 3.18 mg ml(-1) and for oat spelt xylan 6.45 mg ml(-1), while the Km value of xylanase II for birch wood xylan was 6.51 mg ml(-1) and for oat spelt xylan 7.69 mg ml(-1). Metal ions like Al3+, Ba2+, Ca2+, Na+, and Zn2+ enhanced the activity of xylanase I and II at 10 mM concentration. Among the additives, L-tryptophan enhanced the activity of xylanase I and II at 10-, 20-, and 30-mM concentrations. Both xylanases appeared to be glycoproteins.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0273-2289
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
149
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
229-43
|
| pubmed:meshHeading |
pubmed-meshheading:18500584-Aspergillus,
pubmed-meshheading:18500584-Chromatography, Gel,
pubmed-meshheading:18500584-Chromatography, Ion Exchange,
pubmed-meshheading:18500584-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:18500584-Kinetics,
pubmed-meshheading:18500584-Substrate Specificity,
pubmed-meshheading:18500584-Temperature,
pubmed-meshheading:18500584-Xylosidases
|
| pubmed:year |
2008
|
| pubmed:articleTitle |
Purification and biochemical characterization of two xylanases from Aspergillus sydowii SBS 45.
|
| pubmed:affiliation |
School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India. suprabha.nair@gmail.com
|
| pubmed:publicationType |
Journal Article
|