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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4-5
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pubmed:dateCreated |
2008-6-16
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pubmed:abstractText |
A novel method for the quantitation of yonkenafil, a new synthetic phosphodiesterase V inhibitor, in rat plasma using high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS) has been developed. The analyte and internal standard (diazepam) were extracted from plasma (100 microl) by liquid-liquid extraction and separated on a C18 column using 10mM ammonium acetate buffer: methanol (15:85, v/v) as mobile phase in a run time of 3.0 min. The detector was a Q-trap mass spectrometer with an ESI interface operating in the multiple reaction monitoring (MRM) mode. The assay was linear over the concentration range 1.0-1000 ng/ml with a limit of detection of 0.20 ng/ml. Intra- and inter-day precision (as relative standard deviation) were both within 8.45% with good accuracy. The method was successfully applied to a preclinical pharmacokinetic study of yonkenafil in rat after sublingual, oral and intravenous administration. The results demonstrate that the sublingual route gives a higher bioavailablity than the oral route and may represent a useful alternative route of yonkenafil administration.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0731-7085
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
985-9
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:18499386-Acetates,
pubmed-meshheading:18499386-Administration, Oral,
pubmed-meshheading:18499386-Administration, Sublingual,
pubmed-meshheading:18499386-Animals,
pubmed-meshheading:18499386-Biological Availability,
pubmed-meshheading:18499386-Buffers,
pubmed-meshheading:18499386-Chromatography, Liquid,
pubmed-meshheading:18499386-Cyclic Nucleotide Phosphodiesterases, Type 5,
pubmed-meshheading:18499386-Drug Evaluation, Preclinical,
pubmed-meshheading:18499386-Drug Stability,
pubmed-meshheading:18499386-Fasting,
pubmed-meshheading:18499386-Hydrogen-Ion Concentration,
pubmed-meshheading:18499386-Injections, Intravenous,
pubmed-meshheading:18499386-Male,
pubmed-meshheading:18499386-Methanol,
pubmed-meshheading:18499386-Molecular Structure,
pubmed-meshheading:18499386-Phosphodiesterase 5 Inhibitors,
pubmed-meshheading:18499386-Phosphodiesterase Inhibitors,
pubmed-meshheading:18499386-Quality Control,
pubmed-meshheading:18499386-Random Allocation,
pubmed-meshheading:18499386-Rats,
pubmed-meshheading:18499386-Rats, Wistar,
pubmed-meshheading:18499386-Reference Standards,
pubmed-meshheading:18499386-Reproducibility of Results,
pubmed-meshheading:18499386-Sensitivity and Specificity,
pubmed-meshheading:18499386-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:18499386-Tandem Mass Spectrometry,
pubmed-meshheading:18499386-Time Factors
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pubmed:year |
2008
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pubmed:articleTitle |
A rapid and sensitive LC-MS/MS assay to quantify yonkenafil in rat plasma with application to preclinical pharmacokinetics studies.
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pubmed:affiliation |
Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130023, China.
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pubmed:publicationType |
Journal Article,
Evaluation Studies
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