Source:http://linkedlifedata.com/resource/pubmed/id/18498706
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2008-5-23
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| pubmed:abstractText |
In this work, liquid chromatography-electrospray ionization tandem ion-trap mass spectrometry (LC-MS(n)) was used to investigate the in-vivo and in-vitro metabolism of tectoridin. After oral administration of a single dose (100 mg kg(-1)) of tectoridin to healthy rats, faeces and urine samples were collected for 0-48 h and 0-24 h, respectively. Tectoridin was also incubated with rat intestinal flora and rat liver microsomes. Samples from in-vivo and in-vitro metabolism studies were purified using a C(18) solid-phase extraction cartridge, then separated using a reverse-phase C(18) column with methanol/ water (30:70, v/v, adjusted to pH 10.0 with ammonia water) as mobile phase and detected by an on-line MS(n) system. The structure of the metabolites was elucidated by comparing their molecular weights, retention times and full-scan MS(n) spectra with those of the parent drug. The results revealed six metabolites of tectoridin in urine (tectorigenin, hydrogenated tectorigenin, mono-hydroxylated tectorigenin, di-hydroxylated tectorigenin, glucuronide-conjugated tectorigenin and sulfate-conjugated tectorigenin); three metabolites in faeces (tectorigenin, di-hydroxylated tectorigenin and sulfateconjugated tectorigenin); one metabolite in the intestinal flora incubation mixture (tectorigenin), and four in the liver microsomal incubation mixture (tectorigenin, hydrogenated tectorigenin, mono-hydroxylated tectorigenin and di-hydroxylated tectorigenin). Except for tectorigenin, all other metabolites of tectoridin are reported for the first time.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0022-3573
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
60
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
709-16
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| pubmed:meshHeading |
pubmed-meshheading:18498706-Animals,
pubmed-meshheading:18498706-Chromatography, Liquid,
pubmed-meshheading:18498706-Female,
pubmed-meshheading:18498706-Intestines,
pubmed-meshheading:18498706-Isoflavones,
pubmed-meshheading:18498706-Male,
pubmed-meshheading:18498706-Microsomes, Liver,
pubmed-meshheading:18498706-Rats,
pubmed-meshheading:18498706-Rats, Wistar,
pubmed-meshheading:18498706-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:18498706-Tandem Mass Spectrometry
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| pubmed:year |
2008
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| pubmed:articleTitle |
Identification of metabolites of tectoridin in-vivo and in-vitro by liquid chromatography-tandem mass spectrometry.
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| pubmed:affiliation |
Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine, Hubei University, 430062, P. R. China.
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| pubmed:publicationType |
Journal Article
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