Linked Life Data

18487606

Source:http://linkedlifedata.com/resource/pubmed/id/18487606

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
29
pubmed:dateCreated
2008-7-14
pubmed:abstractText
Nucleoside 2'-deoxyribosyltransferases catalyze the transfer of 2-deoxyribose between bases and have been widely used as biocatalysts to synthesize a variety of nucleoside analogs. The genes encoding nucleoside 2'-deoxyribosyltransferase (ndt) from Lactobacillus leichmannii and Lactobacillus fermentum underwent random mutagenesis to select variants specialized for the synthesis of 2',3'-dideoxynucleosides. An Escherichia coli strain, auxotrophic for uracil and unable to use 2',3'-dideoxyuridine, cytosine, and 2',3'-dideoxycytidine as a source of uracil was constructed. Randomly mutated lactobacilli ndt libraries from two species, L. leichmannii and L. fermentum, were screened for the production of uracil with 2',3'-dideoxyuridine as a source of uracil. Several mutants suitable for the synthesis of 2',3'-dideoxynucleosides were isolated. The nucleotide sequence of the corresponding genes revealed a single mutation (G --> A transition) leading to the substitution of a small aliphatic amino acid by a nucleophilic one, A15T (L. fermentum) or G9S (L. leichmannii), respectively. We concluded that the "adaptation" of the nucleoside 2'-deoxyribosyltransferase activity to 2,3-dideoxyribosyl transfer requires an additional hydroxyl group on a key amino acid side chain of the protein to overcome the absence of such a group in the corresponding substrate. The evolved proteins also display significantly improved nucleoside 2',3'-didehydro-2',3'-dideoxyribosyltransferase activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
283
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
20053-9
pubmed:meshHeading
pubmed-meshheading:18487606-Amino Acid Sequence, pubmed-meshheading:18487606-Amino Acid Substitution, pubmed-meshheading:18487606-Catalytic Domain, pubmed-meshheading:18487606-Hydrogen, pubmed-meshheading:18487606-Kinetics, pubmed-meshheading:18487606-Lactobacillus fermentum, pubmed-meshheading:18487606-Lactobacillus leichmannii, pubmed-meshheading:18487606-Models, Molecular, pubmed-meshheading:18487606-Molecular Sequence Data, pubmed-meshheading:18487606-Mutation, pubmed-meshheading:18487606-Nucleosides, pubmed-meshheading:18487606-Oxygen, pubmed-meshheading:18487606-Pentosyltransferases, pubmed-meshheading:18487606-Protein Engineering, pubmed-meshheading:18487606-Protein Structure, Tertiary, pubmed-meshheading:18487606-Sequence Alignment, pubmed-meshheading:18487606-Substrate Specificity
pubmed:year
2008
pubmed:articleTitle
In vivo reshaping the catalytic site of nucleoside 2'-deoxyribosyltransferase for dideoxy- and didehydronucleosides via a single amino acid substitution.
pubmed:affiliation
Institut Pasteur, Unité de Chimie Organique, CNRS, URA2128, Paris Cedex 15, France. akaminsk@pasteur.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't