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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2008-9-1
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pubmed:abstractText |
The nasal epithelium is continuously subjected to wall shear stresses (WSS) induced by respiratory airflows. An in vitro experimental model was developed to expose nasal epithelial cells cultured under air-liquid interface conditions to steady airflow-induced WSS. Mucus secretion from epithelial goblet cells was quantified using an enzyme-linked lectinosorbent assay, and modifications of the cytoskeletal structure were qualitatively evaluated from fluorescent stains of actin and beta-tubulin fibers. The results show increased mucus secretion from cells subjected to WSS of 0.1 and 1.0 dyne/cm(2) for more than 15 min in comparison with unstressed cells. The integrity levels of beta-tubulin fibers were significantly lower in cells subjected to WSS than in unstressed cells. The increased mucus secretion in response to WSS was approximately the same in Taxol-free and Taxol-treated cultures, which indicates that there is no direct connection between beta-tubulin fragmentation and mucus secretion. The stressed cells regained their normal cytoskeletal appearance 24 h after the exposure to WSS. The results of this study suggest that WSS have an important role in the mechanical regulation of the nasal surface epithelium function.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18487304-10591386,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1542-0086
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2998-3008
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:18487304-Animals,
pubmed-meshheading:18487304-Biomechanics,
pubmed-meshheading:18487304-Cattle,
pubmed-meshheading:18487304-Cells, Cultured,
pubmed-meshheading:18487304-Cytoskeleton,
pubmed-meshheading:18487304-Humans,
pubmed-meshheading:18487304-Mucus,
pubmed-meshheading:18487304-Nasal Cavity,
pubmed-meshheading:18487304-Nasal Mucosa,
pubmed-meshheading:18487304-Pulmonary Ventilation,
pubmed-meshheading:18487304-Respiration
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pubmed:year |
2008
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pubmed:articleTitle |
Mucus secretion and cytoskeletal modifications in cultured nasal epithelial cells exposed to wall shear stresses.
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pubmed:affiliation |
Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel. nuritet@eng.tau.ac.il
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pubmed:publicationType |
Journal Article
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