Linked Life Data

18487207

Source:http://linkedlifedata.com/resource/pubmed/id/18487207

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
28
pubmed:dateCreated
2008-7-8
pubmed:abstractText
In human cancers, mutations in components of the Wnt signaling pathway lead to beta-catenin stabilization and result in augmented gene transcription. HCT116 colon cancer cells carry stabilizing mutations in beta-catenin and exhibit an elevated activation of Wnt signaling. To clarify the role of an overactive Wnt signaling, we used DNA microarray analysis to search for genes whose expression is up-regulated after knockdown of the wild type adenomatous polyposis coli (APC) tumor suppressor in HCT116 cells, which further enhances Wnt signaling activation. Serum and glucocorticoid-inducible kinase 1 (SGK1) was among the most up-regulated genes following APC knockdown through small interfering RNA. Up-regulation of SGK1 in response to small interfering RNA against APC was inhibited by concomitant knockdown of beta-catenin. Quantitative real time reverse transcription-PCR, Western blot, and chromatin immunoprecipitation analyses confirmed that SGK1 is a direct beta-catenin target gene. SGK1 negatively regulates the pro-apoptotic transcription factor Forkhead box O3a (FoxO3a) via phosphorylation and exclusion from the nucleus. We show that Wnt signaling activation results in FoxO3a exclusion from the nucleus and inhibits expression of FoxO3a target genes. Importantly, FoxO3a mutants that fail to be phosphorylated and therefore are regulated by SGK1 are not influenced by activation of Wnt signaling. In line, knockdown of SGK1 relieves the effects of Wnt signaling on FoxO3a localization and FoxO3a-dependent transcription. Finally, we show that induction of Wnt signaling inhibits FoxO3a-induced apoptosis. Collectively our results indicate that evasion of apoptosis is another feature employed by an overactive Wnt signaling.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
283
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19201-10
pubmed:dateRevised
2011-11-2
pubmed:meshHeading
pubmed-meshheading:18487207-Active Transport, Cell Nucleus, pubmed-meshheading:18487207-Adenomatous Polyposis Coli Protein, pubmed-meshheading:18487207-Apoptosis, pubmed-meshheading:18487207-Cell Line, Tumor, pubmed-meshheading:18487207-Cell Nucleus, pubmed-meshheading:18487207-Colonic Neoplasms, pubmed-meshheading:18487207-Forkhead Transcription Factors, pubmed-meshheading:18487207-Gene Expression Profiling, pubmed-meshheading:18487207-Gene Expression Regulation, Neoplastic, pubmed-meshheading:18487207-Humans, pubmed-meshheading:18487207-Immediate-Early Proteins, pubmed-meshheading:18487207-Mutation, pubmed-meshheading:18487207-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:18487207-Phosphorylation, pubmed-meshheading:18487207-Protein-Serine-Threonine Kinases, pubmed-meshheading:18487207-RNA, Small Interfering, pubmed-meshheading:18487207-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:18487207-Signal Transduction, pubmed-meshheading:18487207-Transcription, Genetic, pubmed-meshheading:18487207-Up-Regulation, pubmed-meshheading:18487207-Wnt Proteins, pubmed-meshheading:18487207-beta Catenin
pubmed:year
2008
pubmed:articleTitle
Wnt signaling inhibits Forkhead box O3a-induced transcription and apoptosis through up-regulation of serum- and glucocorticoid-inducible kinase 1.
pubmed:affiliation
Department of Experimental Medicine II, Nikolaus-Fiebiger-Center for Molecular Medicine, University of Erlangen, Glueckstrasse 6, 91054 Erlangen, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't