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rdf:type |
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lifeskim:mentions |
umls-concept:C0014597,
umls-concept:C0030685,
umls-concept:C0127400,
umls-concept:C0230704,
umls-concept:C0300926,
umls-concept:C0333348,
umls-concept:C0391871,
umls-concept:C0458827,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1363844,
umls-concept:C1963578
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pubmed:issue |
1
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pubmed:dateCreated |
2008-6-16
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pubmed:abstractText |
This report addressed the question whether ExoU stimulation of airway epithelial cells may contribute to the inflammatory response detected in the course of Pseudomonas aeruginosa respiratory infections. Infection with PA103 P. aeruginosa elicited a potent release of IL-6 and IL-8, as well as of arachidonic acid (AA) and PGE(2) that was reduced by the bacterial treatment with MAFP, a cPLA(2) inhibitor. Airway cells from the BEAS-2B line and in primary culture were shown to be enriched in lipid bodies (LBs), that are cytoplasmic domains implicated in AA transformation into eicosanoids. However, cells infected with PA103 and with a mutant deficient in exoU but complemented with a functional gene exhibited reduced contents of LBs, and this reduction was inhibited by MAFP. FACS analysis showed that the decrease in the LB content correlated with the presence of intracellular PGE(2). Also, in PA103-infected cells, PGE(2) was immunolocalized in LBs, suggesting that the reduction in the cell content of the organelles was due to consumption of their glycerolipids, resulting in local synthesis of the prostanoid. In conclusion, we showed the ExoU ability to induce airway epithelial cells to overproduce PGE(2) and we speculate that LB may represent intracellular loci involved in ExoU-induced eicosanoid synthesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0882-4010
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30-7
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pubmed:meshHeading |
pubmed-meshheading:18486438-Arachidonic Acid,
pubmed-meshheading:18486438-Arachidonic Acids,
pubmed-meshheading:18486438-Bacterial Proteins,
pubmed-meshheading:18486438-Bronchi,
pubmed-meshheading:18486438-Cell Line,
pubmed-meshheading:18486438-Cytokines,
pubmed-meshheading:18486438-Enzyme Inhibitors,
pubmed-meshheading:18486438-Epithelial Cells,
pubmed-meshheading:18486438-Humans,
pubmed-meshheading:18486438-Inclusion Bodies,
pubmed-meshheading:18486438-Inflammation Mediators,
pubmed-meshheading:18486438-Lipid Metabolism,
pubmed-meshheading:18486438-Phosphonic Acids,
pubmed-meshheading:18486438-Prostaglandins E,
pubmed-meshheading:18486438-Pseudomonas Infections,
pubmed-meshheading:18486438-Pseudomonas aeruginosa
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pubmed:year |
2008
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pubmed:articleTitle |
Lipid body mobilization in the ExoU-induced release of inflammatory mediators by airway epithelial cells.
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pubmed:affiliation |
Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Avenue 28 de Setembro, 87 Fundos, 3o andar, 20 551-030, Rio de Janeiro, Brazil. crisplot@yahoo.com.br
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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