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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1991-4-22
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pubmed:abstractText |
Heat shock treatment of rat embryo fibroblasts resulted in a 60% increase in cytosolic protein kinase C activity, in contrast to phorbol ester-induced translocation to the membrane. During reversal of the cells back to the normal temperature a decrease in cytosolic PKC activity was observed and paralleled by an increase in protamine kinase activity. Cell lysates prepared from heat shock-treated cells show a marked calcium/phospholipid-dependent phosphorylation of several endogenous PKC substrate proteins, while the 28-kDa stress protein was shown to be a PKC substrate. These cells express the TYPE III-alpha isoform of PKC and, thus, the alterations induced within cells exposed to hyperthermic treatment may reflect a functional significance with regard to the regulation of this specific isoform.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0014-4827
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
193
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
274-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1848515-Animals,
pubmed-meshheading:1848515-Cell Line,
pubmed-meshheading:1848515-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:1848515-Heat-Shock Proteins,
pubmed-meshheading:1848515-Hot Temperature,
pubmed-meshheading:1848515-Isoenzymes,
pubmed-meshheading:1848515-Molecular Weight,
pubmed-meshheading:1848515-Phosphoproteins,
pubmed-meshheading:1848515-Protamine Kinase,
pubmed-meshheading:1848515-Protein Kinase C,
pubmed-meshheading:1848515-Protein Kinases,
pubmed-meshheading:1848515-Rats
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pubmed:year |
1991
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pubmed:articleTitle |
Alterations in protein kinase C type III-alpha during heat shock of rat embryo fibroblasts.
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pubmed:affiliation |
Auburn University, Department of Zoology & Wildlife Science, Alabama 36849-5414.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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