Linked Life Data

18485060

Source:http://linkedlifedata.com/resource/pubmed/id/18485060

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2008-9-10
pubmed:abstractText
SUMMARY: Ubiquitin-specific proteases (UBPs) are a highly conserved family of proteins in eukaryotes, and play critical roles in protein de-ubiquitination. Here we report a systematic genetic and expression profiling analysis of the UBP gene family in the Arabidopsis thaliana genome. Mutation analysis of 25 of the 27 member genes representing 13 of the 14 sub-families of the UBP gene family revealed that single-gene mutants of three genes in two sub-families exhibit visible phenotypes. Two of these three genes belonging to the UBP15 sub-family were selected for further characterization. The ubp15 mutants display narrower, serrated and flat rosette leaves, partially due to a defect in cell proliferation, as well as other phenotypes such as early flowering, weak apical dominance and reduced fertility, while the line over-expressing UBP15 shows opposite phenotypes. We demonstrated that UPB15 has UBP activity in vitro, and that this biochemical activity is essential for its in vivo function. A genetic interaction analysis among members of this sub-family revealed that UBP15 and UBP16, but not UBP17, have functional redundancy. Our data thus suggest that distinct UBPs, even within a closely related sub-family, can function in different developmental pathways. Although there are clearly functional redundancies among related sub-family members, those redundancies cannot be inferred simply based on the amino acid identity of the family members.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1365-313X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
844-56
pubmed:meshHeading
pubmed-meshheading:18485060-Arabidopsis, pubmed-meshheading:18485060-Arabidopsis Proteins, pubmed-meshheading:18485060-DNA, Bacterial, pubmed-meshheading:18485060-Endopeptidases, pubmed-meshheading:18485060-Gene Expression, pubmed-meshheading:18485060-Gene Expression Profiling, pubmed-meshheading:18485060-Genes, Plant, pubmed-meshheading:18485060-Multigene Family, pubmed-meshheading:18485060-Mutagenesis, Insertional, pubmed-meshheading:18485060-Phenotype, pubmed-meshheading:18485060-Phylogeny, pubmed-meshheading:18485060-Plant Leaves, pubmed-meshheading:18485060-Plants, Genetically Modified, pubmed-meshheading:18485060-RNA, Plant, pubmed-meshheading:18485060-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:18485060-Sequence Alignment, pubmed-meshheading:18485060-Ubiquitination
pubmed:year
2008
pubmed:articleTitle
Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development.
pubmed:affiliation
Graduate Program in Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.