pubmed-article:1848487 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:1848487 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
pubmed-article:1848487 | lifeskim:mentions | umls-concept:C0003873 | lld:lifeskim |
pubmed-article:1848487 | lifeskim:mentions | umls-concept:C0039097 | lld:lifeskim |
pubmed-article:1848487 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
pubmed-article:1848487 | lifeskim:mentions | umls-concept:C0039099 | lld:lifeskim |
pubmed-article:1848487 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:1848487 | pubmed:dateCreated | 1991-4-19 | lld:pubmed |
pubmed-article:1848487 | pubmed:abstractText | The dominant presence of specific T-cell populations in the rheumatoid joint as detected by Southern blot analysis of T cell receptor (TCR) gene rearrangements would indicate local antigen recognition and T cell proliferation. We therefore studied TCR beta chain gene rearrangements using a C beta 2 probe in paired samples of T cell populations from synovial tissue and peripheral blood (n = 6) as well as synovial fluid (n = 16) and peripheral blood (n = 18) of patients with rheumatoid arthritis (RA). Peripheral blood mononuclear cells from healthy donors (n = 7) served as a control. T cells were studied directly after isolation or after non-specific expansion with OKT3 monoclonal antibody (MoAb) and T cell growth factor (TCGF). DNA samples were digested with EcoRI and HindIII to detect rearrangements to C beta 1 and C beta 2, respectively. Extra bands were detected in all EcoRI-digested DNA samples prepared from both freshly isolated and non-specifically expanded T cell populations of patients and healthy donors, possibly representing 'common' (V-) D-J rearrangements. Dominant rearrangements were found in only two out of 16 synovial fluid T cell populations (one freshly isolated and one expanded) and not in peripheral blood or synovial tissue derived T cell populations. No extra bands were detected in HindIII-digested DNA samples. To investigate the effect of in vitro culture techniques on rearrangement patterns we studied DNA samples prepared from synovial tissue T cells obtained both by outgrowth from tissue with TCGF or by enzyme digestion and subsequent expansion either with TCGF or with OKT3 MoAb and TCGF. Whereas the latter T cell population yielded 'common' rearrangements, the former T cell populations yielded different dominant rearrangements. These data indicate that oligoclonality of the T cell populations in synovial tissue and synovial fluid of patients with RA is a rare event. The data also show the influence of in vitro culture techniques on the result of TCR gene rearrangement analysis. | lld:pubmed |
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pubmed-article:1848487 | pubmed:language | eng | lld:pubmed |
pubmed-article:1848487 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1848487 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:1848487 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:1848487 | pubmed:month | Mar | lld:pubmed |
pubmed-article:1848487 | pubmed:issn | 0009-9104 | lld:pubmed |
pubmed-article:1848487 | pubmed:author | pubmed-author:GageA PAP | lld:pubmed |
pubmed-article:1848487 | pubmed:author | pubmed-author:De VriesR RRR | lld:pubmed |
pubmed-article:1848487 | pubmed:author | pubmed-author:BreedveldF... | lld:pubmed |
pubmed-article:1848487 | pubmed:author | pubmed-author:MiltenburgA... | lld:pubmed |
pubmed-article:1848487 | pubmed:author | pubmed-author:VerdonkM JMJ | lld:pubmed |
pubmed-article:1848487 | pubmed:author | pubmed-author:Van LaarJ MJM | lld:pubmed |
pubmed-article:1848487 | pubmed:author | pubmed-author:Van den... | lld:pubmed |
pubmed-article:1848487 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:1848487 | pubmed:volume | 83 | lld:pubmed |
pubmed-article:1848487 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:1848487 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:1848487 | pubmed:pagination | 352-8 | lld:pubmed |
pubmed-article:1848487 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:1848487 | pubmed:year | 1991 | lld:pubmed |
pubmed-article:1848487 | pubmed:articleTitle | Lack of T cell oligoclonality in enzyme-digested synovial tissue and in synovial fluid in most patients with rheumatoid arthritis. | lld:pubmed |
pubmed-article:1848487 | pubmed:affiliation | Department of Rheumatology, University Hospital, Leiden, The Netherlands. | lld:pubmed |
pubmed-article:1848487 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:1848487 | pubmed:publicationType | Comparative Study | lld:pubmed |
pubmed-article:1848487 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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