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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1991-4-19
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pubmed:abstractText |
The dominant presence of specific T-cell populations in the rheumatoid joint as detected by Southern blot analysis of T cell receptor (TCR) gene rearrangements would indicate local antigen recognition and T cell proliferation. We therefore studied TCR beta chain gene rearrangements using a C beta 2 probe in paired samples of T cell populations from synovial tissue and peripheral blood (n = 6) as well as synovial fluid (n = 16) and peripheral blood (n = 18) of patients with rheumatoid arthritis (RA). Peripheral blood mononuclear cells from healthy donors (n = 7) served as a control. T cells were studied directly after isolation or after non-specific expansion with OKT3 monoclonal antibody (MoAb) and T cell growth factor (TCGF). DNA samples were digested with EcoRI and HindIII to detect rearrangements to C beta 1 and C beta 2, respectively. Extra bands were detected in all EcoRI-digested DNA samples prepared from both freshly isolated and non-specifically expanded T cell populations of patients and healthy donors, possibly representing 'common' (V-) D-J rearrangements. Dominant rearrangements were found in only two out of 16 synovial fluid T cell populations (one freshly isolated and one expanded) and not in peripheral blood or synovial tissue derived T cell populations. No extra bands were detected in HindIII-digested DNA samples. To investigate the effect of in vitro culture techniques on rearrangement patterns we studied DNA samples prepared from synovial tissue T cells obtained both by outgrowth from tissue with TCGF or by enzyme digestion and subsequent expansion either with TCGF or with OKT3 MoAb and TCGF. Whereas the latter T cell population yielded 'common' rearrangements, the former T cell populations yielded different dominant rearrangements. These data indicate that oligoclonality of the T cell populations in synovial tissue and synovial fluid of patients with RA is a rare event. The data also show the influence of in vitro culture techniques on the result of TCR gene rearrangement analysis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2154031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2457140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2479386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2525274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2535861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2548202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2653372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2677142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2784966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2874329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2904267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2955515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2963340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2966470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-2993886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-3263133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-3264771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-3308489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-3875651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-6334237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-6610915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-6751631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-6966664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-6981702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1848487-7386498
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
352-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1848487-Adult,
pubmed-meshheading:1848487-Aged,
pubmed-meshheading:1848487-Aged, 80 and over,
pubmed-meshheading:1848487-Antibodies, Monoclonal,
pubmed-meshheading:1848487-Arthritis, Rheumatoid,
pubmed-meshheading:1848487-Blotting, Southern,
pubmed-meshheading:1848487-Cell Separation,
pubmed-meshheading:1848487-Cells, Cultured,
pubmed-meshheading:1848487-Female,
pubmed-meshheading:1848487-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:1848487-Humans,
pubmed-meshheading:1848487-Hyaluronoglucosaminidase,
pubmed-meshheading:1848487-Interleukin-2,
pubmed-meshheading:1848487-Male,
pubmed-meshheading:1848487-Microbial Collagenase,
pubmed-meshheading:1848487-Middle Aged,
pubmed-meshheading:1848487-Muromonab-CD3,
pubmed-meshheading:1848487-Synovial Fluid,
pubmed-meshheading:1848487-Synovial Membrane,
pubmed-meshheading:1848487-T-Lymphocytes
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pubmed:year |
1991
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pubmed:articleTitle |
Lack of T cell oligoclonality in enzyme-digested synovial tissue and in synovial fluid in most patients with rheumatoid arthritis.
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pubmed:affiliation |
Department of Rheumatology, University Hospital, Leiden, The Netherlands.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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