Linked Life Data

18479066

Source:http://linkedlifedata.com/resource/pubmed/id/18479066

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2008-5-15
pubmed:abstractText
Padlock probe was designed based on the sequence of the unique hypothetic protein gene in complete genome of Xanthomonas axonopodis pv. citri (Xac), and amplification primers ware designed according to the universal linking sequence of padlock probe. Detection system of rolling circle amplification (RCA) was established and optimized. Results show that the system could detect Xac and its DNA specifically, while other plant pathogens and bacteria attached on the surface of citrus leaves could not be detected. This indicates that the detection system had its specificity. The detection sensitivity of RCA was 20 cfu/microL for Xac cells and 10(2) copy/microL for cloned DNA fragment, which was slightly higher than the sensitivity of conventional PCR. Leaf samples collected from orange orchards were detected with both RCA and conventional PCR. The result shows that the Xac positive percentage had no remarkable difference between the two methods (P>0.01). Because the universal linking sequence in padlock probe can use same amplification condition, the new technology and detection system can be used to detect diverse plant pathogens simultaneously in plant quarantine and disease pre-symptom diagnosis.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0001-6209
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
375-9
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
[Detection system for Xanthomonas axonopodis pv. citri using rolling circle amplification].
pubmed:affiliation
Key Laboratory of Gene Function and Regulation in Chongqing, College of Bioengineering at Chongqing University, Chongqing 400030, China. huangguanjun574@163.com
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't