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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1991-4-2
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pubmed:abstractText |
A successful immune response requires intercellular contact between T and B lymphocytes. We recently showed that CD28, a T cell surface protein that regulates an activation pathway, could mediate intercellular adhesion with activated B cells by interaction with the B7 antigen. Here we show that CD28 is the primary receptor for B7 on activated peripheral blood T cells, that CD28 binds to B7 in the absence of other accessory molecules, and that interaction between CD28 and B7 is costimulatory for T cell activation. To characterize the binding of CD28 to B7, we have produced genetic fusions of the extracellular portions of B7 and CD28, and immunoglobulin (Ig) C gamma 1 chains. 125I-labeled B7 Ig bound to CD28-transfected Chinese hamster ovary (CHO) cells, and to immobilized CD28 Ig with a Kd approximately 200 nM. B7 Ig also inhibited CD28-mediated cellular adhesion. The function of CD28-B7 interactions during T cell activation was investigated with soluble fusion proteins and with B7-transfected CHO cells. Immobilized B7 Ig and B7+ CHO cells costimulated T cell proliferation. Stimulation of T cells with B7+ CHO cells also specifically increased levels of interleukin 2 transcripts. These results demonstrate that the CD28 signaling pathway could be activated by B7, resulting in increased T cell cytokine production and T cell proliferation. Cellular interactions mediated by B7 and CD28 may represent an important component of the functional interactions between T and B lymphoid cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-1694723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-1697984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-1971273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2113314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2156582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2162180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2211998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2419912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2437578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2441877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2442611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2454644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2465550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2477485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2536900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2540528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2543930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2647304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2779549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2794510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-2825196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-3021470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-3289580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-3488815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-4104295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-6274961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1847722-6458039
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Mitogen,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
173
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
721-30
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1847722-Animals,
pubmed-meshheading:1847722-Antigens, CD28,
pubmed-meshheading:1847722-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:1847722-Base Sequence,
pubmed-meshheading:1847722-Cell Line,
pubmed-meshheading:1847722-Humans,
pubmed-meshheading:1847722-Interleukin-2,
pubmed-meshheading:1847722-Interleukin-4,
pubmed-meshheading:1847722-Kinetics,
pubmed-meshheading:1847722-Lymphocyte Activation,
pubmed-meshheading:1847722-Molecular Sequence Data,
pubmed-meshheading:1847722-Oligonucleotide Probes,
pubmed-meshheading:1847722-Plasmids,
pubmed-meshheading:1847722-Polymerase Chain Reaction,
pubmed-meshheading:1847722-RNA, Messenger,
pubmed-meshheading:1847722-Receptors, Interleukin-4,
pubmed-meshheading:1847722-Receptors, Mitogen,
pubmed-meshheading:1847722-Recombinant Fusion Proteins,
pubmed-meshheading:1847722-T-Lymphocytes,
pubmed-meshheading:1847722-Transcription, Genetic,
pubmed-meshheading:1847722-Transfection
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pubmed:year |
1991
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pubmed:articleTitle |
Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation.
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pubmed:affiliation |
Oncogen Division, Bristol-Myers-Squibb Pharmaceutical Research Institute, Seattle, Washington 91821.
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pubmed:publicationType |
Journal Article
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