Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2008-5-14
pubmed:abstractText
P53 mutation has been reported in various solid tumors, acute leukemia and myelodysplastic syndrome (MDS), but the diagnostic significance of p53 in MDS remains to be determined. The purpose of the present paper was to examine p53 mutation and immunostaining of the same patients, because there have been few reports of simultaneous analysis of these markers. Seven p53 mutations were observed among 37 MDS and 11 cases of overt leukemia transformed from MDS (MDS-OL). Mutated p53 mainly observed in high-risk MDS had more intense p53 staining than in MDS with wild-type p53 overexpression. Aplastic anemia (AA) produced no p53 staining. The percentage of p53 staining in MDS (71%) was higher than that of mutated p53 (11%) but did not reach 100% of MDS cases studied, therefore the authors attempted to differentiate MDS, especially refractory anemia (RA) and AA, using a combination of p53 immunostaining, hemoglobin F (HbF) immunostaining and chromosome abnormality, because HbF of erythroblasts was reportedly observed in MDS RA but not in AA. Most MDS/MDS-OL (47/48) had at least one positive marker. Among 11 AA cases, only two were positive for HbF. The present results suggest that the combination of these three markers is useful to discriminate MDS from AA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1440-1827
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
353-60
pubmed:meshHeading
pubmed-meshheading:18477214-Adult, pubmed-meshheading:18477214-Aged, pubmed-meshheading:18477214-Aged, 80 and over, pubmed-meshheading:18477214-Anemia, Aplastic, pubmed-meshheading:18477214-Anemia, Refractory, pubmed-meshheading:18477214-Biological Markers, pubmed-meshheading:18477214-Bone Marrow Cells, pubmed-meshheading:18477214-DNA, Neoplasm, pubmed-meshheading:18477214-DNA Mutational Analysis, pubmed-meshheading:18477214-Diagnosis, Differential, pubmed-meshheading:18477214-Female, pubmed-meshheading:18477214-Fetal Hemoglobin, pubmed-meshheading:18477214-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:18477214-Humans, pubmed-meshheading:18477214-Karyotyping, pubmed-meshheading:18477214-Male, pubmed-meshheading:18477214-Middle Aged, pubmed-meshheading:18477214-Mutation, pubmed-meshheading:18477214-Myelodysplastic Syndromes, pubmed-meshheading:18477214-Tumor Suppressor Protein p53
pubmed:year
2008
pubmed:articleTitle
Characterization of myelodysplastic syndrome and aplastic anemia by immunostaining of p53 and hemoglobin F and karyotype analysis: differential diagnosis between refractory anemia and aplastic anemia.
pubmed:affiliation
Department of Laboratory Medicine, Nagoya University School of Medicine, Nagoya University School of Health Sciences, Nagoya, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't