Linked Life Data

18476819

Source:http://linkedlifedata.com/resource/pubmed/id/18476819

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-5-14
pubmed:abstractText
Cellular interactions in development of the kidney are used as a model of reciprocal inductive events between epithelium and mesenchyme. Time- and labor-intensive methods have been developed to study this phenomenon. For example, in mice, the targeted disruption of genes in vivo has been used to modify the genetic program directing kidney development. However, gene targeting is a resource-intensive approach and alternative strategies for gene and protein modification in the kidney need to be developed. Herein, we have developed an efficient system for the delivery of antisense morpholino to alter normal protein expression. We describe the use of Endo-Porter to effectively deliver morpholinos to all parts and regions of the kidney explant. Also, we definitively show via confocal microscopy and Western blot analysis that the use of Endo-Porter in delivering antisense morpholinos is robust throughout the entire kidney explant, providing efficient suppression of protein expression. This method saves time and cost when compared with targeted disruption and is an improvement upon previous kidney organ culture methods.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-10322632, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-10600941, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-10985348, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-12190985, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-12882970, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-13111219, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-14645201, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-14718543, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-16107717, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-16394126, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-7129481, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-8812115, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-9149841, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-9507058, http://linkedlifedata.com/resource/pubmed/commentcorrection/18476819-9853247
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0736-6205
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
547-9
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
The use of Endo-Porter to deliver morpholinos in kidney organ culture.
pubmed:affiliation
Maine Medical Center Research Institute, Center for Molecular Medicine, Scarborough, Maine 04074, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural