Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1991-3-26
|
| pubmed:abstractText |
T-cell populations were investigated in the blood and cerebrospinal fluid of patients with multiple sclerosis and other neurological diseases. Individual T cells were directly cloned from the cerebrospinal fluid and blood before in vitro expansion, and their clonotypes were compared by Southern blot analysis of the rearrangement patterns of their T-cell receptor beta chain and gamma chain genes. This allowed the determination of whether two T cell clones shared the same T-cell receptor and thus arose from identical, clonally expanded (oligoclonal) progenitor T cells. As an extension of previous studies, oligoclonal T-cell clones were identified in both cerebrospinal fluid and blood populations in 5 of 9 patients with inflammatory demyelinating disease among a total of 486 blood and cerebrospinal fluid T-cell clones. In contrast, no clonally expanded T-cell populations were found among a total of 424 clones derived from either blood of 4 normal control subjects or blood and cerebrospinal fluid of 8 patients with other neurological diseases. Analysis of T-cell receptor V beta genes among 4 oligoclonal T-cell populations derived from 3 patients with multiple sclerosis demonstrated common usage of the V beta 12 gene segment. These data suggest that oligoclonal T cells share similar specificities and that clonal expansion may have resulted from specific stimulation by an antigen. Moreover, identical clones between blood and cerebrospinal fluid were observed in 3 of 9 patients with demyelinating disease, thus providing further evidence of an equilibrium between peripheral and central nervous system immune compartments.
|
| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0364-5134
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
29
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
33-40
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1847614-Adolescent,
pubmed-meshheading:1847614-Adult,
pubmed-meshheading:1847614-Aged,
pubmed-meshheading:1847614-Base Sequence,
pubmed-meshheading:1847614-Blotting, Southern,
pubmed-meshheading:1847614-Child, Preschool,
pubmed-meshheading:1847614-Clone Cells,
pubmed-meshheading:1847614-DNA Probes,
pubmed-meshheading:1847614-Female,
pubmed-meshheading:1847614-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:1847614-Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor,
pubmed-meshheading:1847614-Humans,
pubmed-meshheading:1847614-Male,
pubmed-meshheading:1847614-Middle Aged,
pubmed-meshheading:1847614-Molecular Sequence Data,
pubmed-meshheading:1847614-Multiple Sclerosis,
pubmed-meshheading:1847614-Phenotype,
pubmed-meshheading:1847614-Polymerase Chain Reaction,
pubmed-meshheading:1847614-T-Lymphocytes
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Common T-cell receptor V beta usage in oligoclonal T lymphocytes derived from cerebrospinal fluid and blood of patients with multiple sclerosis.
|
| pubmed:affiliation |
Department of Medicine, Brigham and Women's Hospital, Boston, MA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|