Linked Life Data

1847356

Source:http://linkedlifedata.com/resource/pubmed/id/1847356

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1991-3-22
pubmed:abstractText
Transformation of retinal neuro-epithelial cells by Rous sarcoma virus (RSV) leads to many alterations in cell phenotype, including changes in cell movement, cell-cell adhesion and protease secretion. To define and quantitate the alterations in cell movement, we analyzed video recordings of cultured cells using the computer-assisted Dynamic Morphology System (DMS). Control neuro-epithelial cells showed very low levels of translocation and membrane activity. After transformation, neuro-epithelial cells exhibited increased membrane activity, although directed cell translocation remained low. Developing retinas also contain a small proportion of Müller glial cells, which were purified by repeated passaging of control cultures. In contrast to neuro-epithelial cells, both control and RSV-transformed glial cells showed high levels of translocation and membrane activity. To analyze how different kinds of cell movement affect invasive behavior, we compared the ability of control and RSV-transformed cells to invade the chorio-allantoic membrane of developing chicken embryos. Control neuro-epithelial cells were not invasive. RSV-transformed neuro-epithelial cells, which showed low levels of translocation as revealed by DMS, were invasive. Similarly, RSV-transformed glial cells were invasive while control glial cells, which translocated, were not invasive. These results suggest that high levels of cell translocation are not necessary for invasion. In addition, the results suggest that elevated membrane activity in neuro-epithelial cells may be important for their invasion.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0020-7136
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
560-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Invasion of Rous sarcoma virus-transformed retinal cells: role of cell motility.
pubmed:affiliation
Department of Anatomy and Cell Biology, University of Cincinnati Medical Center, OH 45267-0521.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.