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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1991-3-15
|
| pubmed:abstractText |
Previous work with membrane vesicles has demonstrated an absolute dependence on K+ for proton translocation by the inorganic pyrophosphatase (H(+)-PPase: EC 3.6.1.1) from the vacuolar membrane (tonoplast) of higher plants. Using intact vacuoles from sugar beet (Beta vulgaris) storage tissue, we have monitored PP1-dependent currents by patch clamp in 'whole vacuole' mode. Serial K+ substitutions were made at both tonoplast faces. The results show that K+ activation occurs only at the cytosolic face.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0014-5793
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
14
|
| pubmed:volume |
278
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
66-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1847114-Biological Transport,
pubmed-meshheading:1847114-Inorganic Pyrophosphatase,
pubmed-meshheading:1847114-Membrane Potentials,
pubmed-meshheading:1847114-Plants,
pubmed-meshheading:1847114-Potassium,
pubmed-meshheading:1847114-Protons,
pubmed-meshheading:1847114-Pyrophosphatases,
pubmed-meshheading:1847114-Vacuoles
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Vacuolar proton-pumping pyrophosphatase in Beta vulgaris shows vectorial activation by potassium.
|
| pubmed:affiliation |
Biology Department, University of York, UK.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|