|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
2008-6-11
|
|
pubmed:abstractText |
Mechanical overloading is a major causative factor of tendinopathy; however, its underlying mechanisms are unclear. We hypothesized mechanical overloading would damage tendons and alter genes associated with tendinopathy in a load-dependent manner. To test this hypothesis, we fatigue loaded rat patellar tendons in vivo and measured expression of the matrix-degrading enzyme MMP-13 and the inflammatory cytokine IL-1beta. We also examined these responses in cultured tenocytes exposed to intermittent hydrostatic pressure in vitro. Additionally, we hypothesized load-induced changes in tenocyte MMP-13 expression would be dependent on expression of IL-1beta. In vivo fatigue loading at 1.7% strain caused overt microstructural damage and upregulated expression of MMP-13 and IL-1beta, while 0.6% strain produced only minor changes in matrix microstructure and downregulated expression of both MMP-13 and IL-1beta. Loading of cultured tenocytes at 2.5 and 7.5 MPa produced comparable changes in expression to those of in vivo tendon loading. Blocking IL-1beta expression with siRNA suppressed load-induced both MMP-13 mRNA expression and activity. The data suggest fatigue loading alters expression of MMP-13 and IL-1beta in tendons in vivo and tenocytes in vitro in a load-dependent manner. The data also suggest MMP-13 is regulated by both IL-1beta-dependent and IL-1beta-independent pathways.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-10367022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-10415734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-10418074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-10810684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-10861084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-11263775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-11307072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-11685366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-11696985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-11852234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-11853088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-11858805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-12009332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-12010565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-12507595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-12553513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-12568957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-12756303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-12761889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-12867575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-12960175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-14504032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-14504039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-14560540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-14977670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-15187863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-15262646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-15325639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-15533922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-15634833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-16256123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-16462458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-16479573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-17054770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-17076612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-17149746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-17293464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-17328060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-17457818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-1748700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-8388071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-8798568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-9211612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-9302611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18470577-9607566
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
1528-1132
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
466
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1555-61
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:18470577-Animals,
pubmed-meshheading:18470577-Biomechanics,
pubmed-meshheading:18470577-Cumulative Trauma Disorders,
pubmed-meshheading:18470577-Disease Models, Animal,
pubmed-meshheading:18470577-Female,
pubmed-meshheading:18470577-Interleukin-1beta,
pubmed-meshheading:18470577-Matrix Metalloproteinase 13,
pubmed-meshheading:18470577-Patellar Ligament,
pubmed-meshheading:18470577-Rats,
pubmed-meshheading:18470577-Rats, Sprague-Dawley,
pubmed-meshheading:18470577-Rupture,
pubmed-meshheading:18470577-Tendinopathy
|
|
pubmed:year |
2008
|
|
pubmed:articleTitle |
Coordinate regulation of IL-1beta and MMP-13 in rat tendons following subrupture fatigue damage.
|
|
pubmed:affiliation |
Leni and Peter May Department of Orthopaedics, Mount Sinai School of Medicine, 5 East 98th Street, 9th Floor, New York, NY 10029, USA.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
