Linked Life Data

18466770

Source:http://linkedlifedata.com/resource/pubmed/id/18466770

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2008-6-3
pubmed:abstractText
We report on the cDNA cloning and characterization of a novel short-chain isoprenyl diphosphate synthase from the aphid Myzus persicae. Of the three IPPS cDNAs we cloned, two yielded prenyltransferase activity following expression in Escherichia coli; these cDNAs encode identical proteins except for the presence, in one of them, of an N-terminal mitochondrial targeting peptide. Although the aphid enzyme was predicted to be a farnesyl diphosphate synthase by BLASTP analysis, rMpIPPS, when isopentenyl diphosphate and dimethylallyl diphosphate are supplied as substrates, typically generated geranyl diphosphate (C10) as its main product, along with significant quantities of farnesyl diphosphate (C15). Analysis of an MpIPPS homology model pointed to substitutions that could confer GPP/FPP synthase activity to the aphid enzyme.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
582
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1928-34
pubmed:dateRevised
2008-8-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Characterization of a novel aphid prenyltransferase displaying dual geranyl/farnesyl diphosphate synthase activity.
pubmed:affiliation
Gembloux Agricultural University, Department of Functional and Evolutionary Entomology, Passage des Déportés 2, B-5030 Gembloux, Belgium. entomologie@fsagx.ac.be <entomologie@fsagx.ac.be>
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't