Linked Life Data

18464252

Source:http://linkedlifedata.com/resource/pubmed/id/18464252

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-10-13
pubmed:abstractText
Oxidation of hemoglobin (Hb) to nonfunctional methemoglobin (metHb) is a main challenge for the fabrication of an ideal Hb-based blood substitute. In this study, a novel nonenzymatic reduction and suppression route, combined with a fast prereduction, optimized double emulsion preparation, and a second sustaining postreduction, was developed to control the metHb in Hb-loaded nanoparticles (HbP) with porous microstructure to a desirable level. In the prereduction, the metHb in the raw Hb was effectively reduced from over 90% to 1.2% using sodium dithionite following gel filtration separation. During the preparation, higher the emulsion strength performed, higher was the extent of Hb oxidized. PEGylated polymer and addition of miscible solvent, such as acetonitrile, into the oil phase could pronouncedly suppress metHb formation. The resultant metHb level in HbP under the optimal fabrication was about 5.6%, which could be further reduced to 1.4% by the model reducing agents in human plasma with the help of superoxide dismutatse and catalase system, which are capable of sustaining postreduction. The oxygen dissociation curve of the HbP was close to that of native Hb, indicating that the oxygen-carrying ability of the Hb, despite initially losing this function due to the severe oxidation, recovered and retained well. The results achieved are promising for the fabrication of blood substitutes with controlled metHb level, which can fulfill the binding/delivering oxygen to tissues in vivo for future trials.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1552-4981
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
354-63
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Reduction and suppression of methemoglobin loaded in the polymeric nanoparticles intended for blood substitutes.
pubmed:affiliation
Key Laboratory for Ultrafine Materials of Ministry of Education, Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't