Source:http://linkedlifedata.com/resource/pubmed/id/18461394
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2008-8-11
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| pubmed:databankReference | |
| pubmed:abstractText |
A psychrotrophic bacterium producing a cold-adapted lipase was isolated from the deep-sea sediment of Prydz Bay, Antarctic and identified as a Pseudomonas strain. Determination of the nucleotide sequence of the gene encoding a lipase from Pseudomonas sp. 7323 (lipA) revealed that LipA is composed of 617 amino acid residues with a calculated molecular weight of 64,466 Da. LipA has a GXSXG motif, which is conserved in lipases/esterases and generally contains the active-site serine. The lipase purified from the Escherichia coli transformant (rLipA) by metal-chelating chromatography exhibited the same electrophoretic mobility as did the wild-type lipase (wLipA) purified from strain 7323, and both enzymes were quite similar in physicochemical properties. The optimal temperature and pH value for the lipases activity were 30 degrees C and 9.0, respectively. They were unstable at temperatures above 25 degrees C and only retained half of their highest activity after incubation at 60 degrees C for 5 min. These results indicated that the enzymes were typical alkaline cold-adapted enzymes. Both enzymes were particularly activated by Ca2+. Additionally, the enzymes hydrolyzed p-nitrophenyl caprate and tributyrin at the highest velocity among the other p-nitrophenyl esters and triglycerides.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1436-2228
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
10
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
612-21
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| pubmed:meshHeading |
pubmed-meshheading:18461394-Amino Acid Sequence,
pubmed-meshheading:18461394-Antarctic Regions,
pubmed-meshheading:18461394-Bacterial Proteins,
pubmed-meshheading:18461394-Cloning, Molecular,
pubmed-meshheading:18461394-Cold Temperature,
pubmed-meshheading:18461394-Detergents,
pubmed-meshheading:18461394-Escherichia coli,
pubmed-meshheading:18461394-Gene Expression Regulation, Bacterial,
pubmed-meshheading:18461394-Hydrogen-Ion Concentration,
pubmed-meshheading:18461394-Molecular Sequence Data,
pubmed-meshheading:18461394-Oceans and Seas,
pubmed-meshheading:18461394-Phylogeny,
pubmed-meshheading:18461394-Pseudomonas,
pubmed-meshheading:18461394-RNA, Ribosomal, 16S,
pubmed-meshheading:18461394-Sequence Alignment,
pubmed-meshheading:18461394-Substrate Specificity,
pubmed-meshheading:18461394-Temperature
|
| pubmed:articleTitle |
Molecular cloning and expression of a cold-adapted lipase gene from an Antarctic deep sea psychrotrophic bacterium Pseudomonas sp. 7323.
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| pubmed:affiliation |
Institute for Biomedical Research, Xiamen University, Xiamen, People's Republic of China. jinweizhang@21cn.com
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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