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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1991-2-14
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pubmed:abstractText |
Production of live attenuated oral poliomyelitis vaccine (OPV) requires rigorous neurovirulence safety testing of each vaccine lot, currently carried out in monkeys. It has been reported that a change from 472-U to 472-C in the type 3 OPV RNA is associated with an increased histologic lesion score produced upon intraspinal inoculation of the mutant virus in monkeys. We have developed a method, based on polymerase chain reaction, for measuring the relative abundance of these mutant sequences directly in vaccine preparations and used this method to evaluate the proportion of 472-C in 40 different lots of type 3 OPV. Six vaccine lots that had failed the intraspinal monkey neurovirulence test contained a higher proportion of 472-C than all other lots that had passed this test. OPV type 3 virus containing 472-C was rapidly selected during serial passages in African green monkey kidney cells that are used for manufacturing of the vaccine. We have also found that the wild-type poliovirus type 3 strain Leon/37, from which the vaccine strain was originally derived, contained a mixture of 472-U and 472-C sequences. No other mutations in OPV type 3 RNA have been detected by similar assays at position 2034, also associated with attenuation, or at several other positions reported to be altered in some vaccine preparations. Our results suggest that molecular diagnostics may provide a supplement or a potential alternative to animal testing of live attenuated vaccines.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-13340601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-13629086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-2155504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-2448875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-2536835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-2536836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-2539491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-2539524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-2552175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-2982959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-2986004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-2999980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-3194208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-6202874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-6310508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1846038-6324200
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
199-203
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1846038-Animal Testing Alternatives,
pubmed-meshheading:1846038-Animals,
pubmed-meshheading:1846038-Base Sequence,
pubmed-meshheading:1846038-Cell Line,
pubmed-meshheading:1846038-Cercopithecus aethiops,
pubmed-meshheading:1846038-Haplorhini,
pubmed-meshheading:1846038-Kidney,
pubmed-meshheading:1846038-Molecular Sequence Data,
pubmed-meshheading:1846038-Mutation,
pubmed-meshheading:1846038-Oligonucleotide Probes,
pubmed-meshheading:1846038-Poliovirus,
pubmed-meshheading:1846038-Poliovirus Vaccine, Oral,
pubmed-meshheading:1846038-Polymerase Chain Reaction,
pubmed-meshheading:1846038-RNA, Viral,
pubmed-meshheading:1846038-Vaccines, Attenuated
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pubmed:year |
1991
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pubmed:articleTitle |
Correlation between amount of virus with altered nucleotide sequence and the monkey test for acceptability of oral poliovirus vaccine.
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pubmed:affiliation |
Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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