Linked Life Data

18456006

Source:http://linkedlifedata.com/resource/pubmed/id/18456006

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7-8
pubmed:dateCreated
2008-6-16
pubmed:abstractText
Water-soluble luminescent colloidal quantum dots (QDs) have attracted great attention in biological and medical applications. In particular, for any potential in vivo application, the interaction of QDs with human serum albumin (HSA) is crucial. As a step toward the elucidation of the fate of QDs introduced to organism, the interactions between QDs and HSA were systematically investigated by various spectroscopic techniques under the physiological conditions. It was proved that binding of QDs and HSA is a result of the formation of QDs-HSA complex and electrostatic interactions play a major role in stabilizing the complex. The modified Stern-Volmer quenching constant K(a) at different temperatures and corresponding thermodynamic parameters DeltaH, DeltaG and DeltaS were calculated. Furthermore, the site marker competitive experiments revealed that the binding location of QDs with HSA is around site I, centered at Lys199. The conformational changes of HSA induced by QDs have been analyzed by means of CD and FT-IR. The results suggested that HSA underwent substantial conformational changes at both secondary and tertiary structure levels. The stoichiometry of HSA attached to QDs was obtained by dynamic light scattering (DLS) and zeta-potential.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:volume
1784
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1020-7
pubmed:meshHeading
pubmed:articleTitle
Conformation, thermodynamics and stoichiometry of HSA adsorbed to colloidal CdSe/ZnS quantum dots.
pubmed:affiliation
State Key Laboratory of Virology, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't