Source:http://linkedlifedata.com/resource/pubmed/id/18454548
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
2008-5-22
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pubmed:abstractText |
Diarrhetic shellfish poisoning (DSP) toxins pose a serious health risk for consumers of bivalves and other shellfish, as well as a huge economic burden for the bivalve-producing farmers. In this work, the aim was to utilize a solubilization-based protein-isolation method to produce a low-DSP toxin protein isolate from toxic blue mussels that are unsuitable for the whole shellfish market. A homogenate of whole mussel meat was solubilized at low pH (2.8) or high pH (11.1), followed by centrifugation and reprecipitation of the solubilized mussel proteins at the isoelectric pH. In a second centrifugation, precipitated proteins were collected. These processes resulted in 81 (acid solubilization) and 72% (alkaline solubilization) reduction in the initial DTX-1 toxin content of the mussel meat. No other DSP toxins were found in the protein isolates. Acid processing of mussel meat resulted in 50% reduction in the total lipid content, while alkaline treatment did not significantly affect the lipid content. The effect of citric acid and calcium chloride addition to the mussel meat-water homogenate on lipid and toxin content was also investigated. A poor correlation factor was surprisingly obtained between reductions in DTX-1 toxin and lipids in protein isolates from processed toxic mussels. Results from an analytical mass balance of the DTX-1 toxin during acid processing showed that 61% of this toxin ended up in the aqueous supernatant after the second centrifugation. The present study presents a promising alternative way of utilizing mussels for food production in periods when they are toxic.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Citric Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Marine Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrans,
http://linkedlifedata.com/resource/pubmed/chemical/dinophysistoxin 1
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-8561
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3675-81
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pubmed:meshHeading |
pubmed-meshheading:18454548-Animals,
pubmed-meshheading:18454548-Calcium Chloride,
pubmed-meshheading:18454548-Citric Acid,
pubmed-meshheading:18454548-Dinoflagellida,
pubmed-meshheading:18454548-Food Handling,
pubmed-meshheading:18454548-Hydrogen-Ion Concentration,
pubmed-meshheading:18454548-Lipids,
pubmed-meshheading:18454548-Marine Toxins,
pubmed-meshheading:18454548-Mytilus edulis,
pubmed-meshheading:18454548-Proteins,
pubmed-meshheading:18454548-Pyrans
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pubmed:year |
2008
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pubmed:articleTitle |
Removal of lipids and diarrhetic shellfish poisoning toxins from blue mussels (Mytilus edulis) during acid and alkaline isolation of proteins.
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pubmed:affiliation |
Department of Chemical and Biological Engineering, Food Science, Chalmers University of Technology, SE 402 29, Gothenburg, Sweden. patroklo@chalmers.se
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pubmed:publicationType |
Journal Article
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