Source:http://linkedlifedata.com/resource/pubmed/id/18453623
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
2008-5-5
|
| pubmed:abstractText |
Non-disrupted pieces of primary human lung tumor implanted into NOD-scid IL2Rgamma(null) mice consistently result in successful xenografts in which tissue architecture, including tumor-associated leukocytes, stromal fibroblasts, and tumor cells are preserved for prolonged periods with limited host-vs-graft interference. Human CD45(+) tumor-associated leukocytes within the xenograft are predominantly CD3(+) T cells with fewer CD138(+) plasma cells. The effector memory T cells that had been shown to be quiescent in human lung tumor microenvironments can be activated in situ as determined by the production of human IFN-gamma in response to exogenous IL-12. Plasma cells remain functional as evidenced by production of human Ig. Significant levels of human IFN-gamma and Ig were detected in sera from xenograft-bearing mice for up to 9 wk postengraftment. Tumor-associated T cells were found to migrate from the microenvironment of the xenograft to the lung, liver, and primarily the spleen. At 8 wk postengraftment, a significant portion of cells isolated from the mouse spleens were found to be human CD45(+) cells. The majority of CD45(+) cells were CD3(+) and expressed a phenotype consistent with an effector memory T cell, consisting of CD4(+) or CD8(+) T cells that were CD45RO(+), CD44(+), CD62L(-), and CD25(-). Following adoptive transfer into non-tumor bearing NOD-scid IL2Rgamma(null) mice, these human T cells were found to expand in the spleen, produce IFN-gamma, and maintain an effector memory phenotype. We conclude that the NOD-scid IL2Rgamma(null) tumor xenograft model provides an opportunity to study tumor and tumor-stromal cell interactions in situ for prolonged periods.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0022-1767
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| pubmed:author |
pubmed-author:BankertRichard BRB,
pubmed-author:BarcosMauriceM,
pubmed-author:ConwayThomas FTFJr,
pubmed-author:KelleherRaymond JRJJr,
pubmed-author:ShultzLeonard DLD,
pubmed-author:Simpson-AbelsonMichelle RMR,
pubmed-author:SonnenbergGregory FGF,
pubmed-author:TakitaHiroshiH,
pubmed-author:YokotaSandra JSJ
|
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
180
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
7009-18
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:18453623-Adoptive Transfer,
pubmed-meshheading:18453623-Animals,
pubmed-meshheading:18453623-Antigens, CD45,
pubmed-meshheading:18453623-Carcinoma, Non-Small-Cell Lung,
pubmed-meshheading:18453623-Disease Models, Animal,
pubmed-meshheading:18453623-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:18453623-Flow Cytometry,
pubmed-meshheading:18453623-Humans,
pubmed-meshheading:18453623-Immunohistochemistry,
pubmed-meshheading:18453623-Immunologic Memory,
pubmed-meshheading:18453623-Immunophenotyping,
pubmed-meshheading:18453623-Interferon-gamma,
pubmed-meshheading:18453623-Lung Neoplasms,
pubmed-meshheading:18453623-Lymphocytes, Tumor-Infiltrating,
pubmed-meshheading:18453623-Mice,
pubmed-meshheading:18453623-Mice, Inbred NOD,
pubmed-meshheading:18453623-Mice, SCID,
pubmed-meshheading:18453623-Neoplasm Transplantation,
pubmed-meshheading:18453623-Receptors, Interleukin-2,
pubmed-meshheading:18453623-T-Lymphocyte Subsets,
pubmed-meshheading:18453623-T-Lymphocytes,
pubmed-meshheading:18453623-Transplantation, Heterologous
|
| pubmed:year |
2008
|
| pubmed:articleTitle |
Long-term engraftment and expansion of tumor-derived memory T cells following the implantation of non-disrupted pieces of human lung tumor into NOD-scid IL2Rgamma(null) mice.
|
| pubmed:affiliation |
State University of New York, Department of Microbiology and Immunology and the Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, Buffalo, NY 14214, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|