Linked Life Data

18453536

Source:http://linkedlifedata.com/resource/pubmed/id/18453536

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
27
pubmed:dateCreated
2008-6-30
pubmed:abstractText
Polycomb group (PcG) proteins form two distinct complexes, PRC1 and PRC2, to regulate developmental target genes by maintaining the epigenetic state in cells. PRC2 methylates histone H3 at lysine 27 (H3K27), and PRC1 then recognizes methyl-H3K27 to form repressive chromatin. However, it remains unknown how PcG proteins maintain stable and plastic chromatin during cell division. Here we report that PcG-associated chromatin is reproduced in the G(1) phase in post-mitotic cells and is required for subsequent S phase progression. In dividing cells, H3K27 trimethylation (H3K27Me(3)) marked mitotic chromosome arms where PRC2 (Suz12 and Ezh2) co-existed, whereas PRC1 (Bmi1 and Pc2) appeared in distinct foci in the pericentromeric regions. As each PRC complex was increasingly assembled from mitosis to G(1) phase, PRC1 formed H3K27Me(3)-based chromatin intensively during middle and late G(1) phase; this chromatin was highly resistant to in situ nuclease treatment. Thus, the transition from mitosis to G(1) phase is crucial for PcG-mediated chromatin inheritance. Knockdown of Suz12 markedly reduced the amount of H3K27Me(3) on mitotic chromosomes, and as a consequence, PRC1 foci were not fully transmitted to post-mitotic daughter cells. S phase progression was markedly delayed in these Suz12-knockdown cells. The fact that PcG-associated chromatin is reproduced during post-mitotic G(1) phase suggests the possibility that PcG proteins enable their target chromatin to be remodeled in response to stimuli in the G(1) phase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
283
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
18905-15
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:18453536-Animals, pubmed-meshheading:18453536-Chromatin, pubmed-meshheading:18453536-Chromatin Assembly and Disassembly, pubmed-meshheading:18453536-DNA-Binding Proteins, pubmed-meshheading:18453536-Epigenesis, Genetic, pubmed-meshheading:18453536-G1 Phase, pubmed-meshheading:18453536-HeLa Cells, pubmed-meshheading:18453536-Histone-Lysine N-Methyltransferase, pubmed-meshheading:18453536-Histones, pubmed-meshheading:18453536-Humans, pubmed-meshheading:18453536-Methylation, pubmed-meshheading:18453536-Mice, pubmed-meshheading:18453536-Mitosis, pubmed-meshheading:18453536-Multiprotein Complexes, pubmed-meshheading:18453536-NIH 3T3 Cells, pubmed-meshheading:18453536-Proteins, pubmed-meshheading:18453536-Repressor Proteins, pubmed-meshheading:18453536-S Phase, pubmed-meshheading:18453536-Transcription Factors
pubmed:year
2008
pubmed:articleTitle
Polycomb group protein-associated chromatin is reproduced in post-mitotic G1 phase and is required for S phase progression.
pubmed:affiliation
Department of Regeneration Medicine, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't