Linked Life Data

18453119

Source:http://linkedlifedata.com/resource/pubmed/id/18453119

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-5-5
pubmed:abstractText
Phagocytosis of invading microorganisms by neutrophils is an important early response to infection. Here, we describe protocols designed for the quantitative study of particle internalization and of the subsequent maturation of the newly formed phagosomes using sheep red blood cells as a model target particle and neutrophils as phagocytes. Techniques to label the particles with fluorescent pH-sensitive dyes are presented, along with complement and immunoglobulin opsonization procedures. These labeling techniques, in combination with high-resolution digital imaging, allow for the quantitative assessment of phagocytosis at the single-cell level by bright field and fluorescence microscopy. Lastly, qualitative and quantitative methods of investigating the intraphagosomal pH are presented. We describe the use of a fluorescent weak base that accumulates in acidic cellular compartments and functions as a marker of phagosome acidification, as well as more quantitative phagosomal pH measurements by fluorescence ratio imaging. A brief description of the hardware and software components necessary for digital imaging is also provided.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
412
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
289-300
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Assessment of phagosome formation and maturation by fluorescence microscopy.
pubmed:affiliation
Cell Biology Program, Hospital for Sick Children, Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't