Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-6-2
pubmed:abstractText
Artesunate, a remarkable antimalarial agent, also reveals profound cytotoxic activity. In the present investigation, we compared the anticancer effects of artesunate on three colorectal cancer cell lines and analyzed the relationship between drug sensitivity and malignant phenotype of the tumor cells. The findings are as follows: poorly-differentiated was colorectal cancer cell line CLY showing nuclear beta-catenin accumulation and loss of E-cadherin; moderately-differentiated were Lovo cells with cytoplasmic distribution of the two proteins; and well-differentiated were HT-29 cells with membranous localization of them. Also, both in vitro and in vivo, poorly- or moderately-differentiated CLY and Lovo were more susceptible to artesunate treatment than well-differentiated HT-29. Furthermore, the sensitive response of CLY and Lovo to artesunate was associated with membranous translocation of beta-catenin and increased expression of E-cadherin, which indicated the inhibition of hyperactive Wnt signaling pathway and the reversion of the epithelial to mesenchymal transition, respectively. Due to the vital roles of Wnt pathway and the epithelial to mesenchymal transition in tumor differentiation, we thought artesunate could promote the re-differentiation and apoptosis of colorectal cancer cells by inhibition of hyperactive Wnt pathway and reversion of the epithelial to mesenchymal transition.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
588
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-8
pubmed:meshHeading
pubmed-meshheading:18448095-Adenocarcinoma, pubmed-meshheading:18448095-Animals, pubmed-meshheading:18448095-Antineoplastic Agents, pubmed-meshheading:18448095-Apoptosis, pubmed-meshheading:18448095-Artemisinins, pubmed-meshheading:18448095-Blotting, Western, pubmed-meshheading:18448095-Cadherins, pubmed-meshheading:18448095-Cell Cycle, pubmed-meshheading:18448095-Cell Differentiation, pubmed-meshheading:18448095-Cell Line, Tumor, pubmed-meshheading:18448095-Cell Proliferation, pubmed-meshheading:18448095-Colorectal Neoplasms, pubmed-meshheading:18448095-Female, pubmed-meshheading:18448095-Flow Cytometry, pubmed-meshheading:18448095-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:18448095-Humans, pubmed-meshheading:18448095-Immunohistochemistry, pubmed-meshheading:18448095-Male, pubmed-meshheading:18448095-Mice, pubmed-meshheading:18448095-Mice, Inbred BALB C, pubmed-meshheading:18448095-Mice, Nude, pubmed-meshheading:18448095-Middle Aged, pubmed-meshheading:18448095-Neoplasm Proteins, pubmed-meshheading:18448095-Subcellular Fractions, pubmed-meshheading:18448095-beta Catenin
pubmed:year
2008
pubmed:articleTitle
Differential sensitivity of colorectal cancer cell lines to artesunate is associated with expression of beta-catenin and E-cadherin.
pubmed:affiliation
Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine, Beijing, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't