Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2008-5-13
pubmed:abstractText
Connective tissue damage and angiogenesis are both important features of tumour growth and invasion. Here, we show that endothelial cells maintained on a three-dimensional lattice of intact polymerised collagen formed a monolayer of cells with a cobblestone morphology. When the collagen was exposed to organ culture fluid from human basal cell tumours of the skin (containing a high level of active matrix metalloproteinase-1 (MMP-1)), degradation of the collagen matrix occurred. The major degradation products were the $3 over 4$- and $1 over 4$-sized fragments known to result from the action of MMP-1 on type I collagen. When endothelial cells were maintained on the partially degraded collagen, the cells organised into a network of vascular tubes. Pretreatment of the organ culture fluid with either tissue inhibitor of metalloproteinase-1 (TIMP-1) or neutralising antibody to MMP-1 prevented degradation of the collagen lattice and concomitantly inhibited endothelial cell organisation into the vascular network. Purified (activated) MMP-1 duplicated the effects of skin organ culture fluid, but other enzymes including MMP-9 (gelatinase B), elastase or trypsin failed to produce measurable fragments from intact collagen and also failed to promote vascular tube formation. Together, these studies suggest that damage to the collagenous matrix is itself an important inducer of new vessel formation.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-10471461, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-10601729, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-10682680, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-11238041, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-11245282, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-11535623, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-12164934, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-12713591, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-12929747, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-14581484, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-15175039, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-15545316, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-15563949, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-15717924, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-15911882, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-16004981, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-16269008, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-16306453, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-16453301, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-16723701, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-17167269, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-7622608, http://linkedlifedata.com/resource/pubmed/commentcorrection/18443597-7743355
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1532-1827
pubmed:author
pubmed:issnType
Electronic
pubmed:day
20
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1646-52
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Vascular tube formation on matrix metalloproteinase-1-damaged collagen.
pubmed:affiliation
Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA. varani@med.umich.edu
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural