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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0023434,
umls-concept:C0185117,
umls-concept:C0205217,
umls-concept:C0683598,
umls-concept:C0876956,
umls-concept:C0936012,
umls-concept:C0939930,
umls-concept:C1122976,
umls-concept:C1539386,
umls-concept:C1539721,
umls-concept:C1880177,
umls-concept:C2911684
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pubmed:issue |
2
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pubmed:dateCreated |
2008-7-8
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pubmed:abstractText |
To identify novel genes involved in the molecular pathogenesis of chronic lymphocytic leukemia (CLL) we performed a serial analysis of gene expression (SAGE) in CLL cells, and compared this with healthy B cells (nCD19(+)). We found a high level of similarity among CLL subtypes, but a comparison of CLL versus nCD19(+) libraries revealed 55 genes that were over-represented and 49 genes that were down-regulated in CLL. A gene ontology analysis revealed that TOSO, which plays a functional role upstream of Fas extrinsic apoptosis pathway, was over-expressed in CLL cells. This finding was confirmed by real-time reverse transcription-polymerase chain reaction in 78 CLL and 12 nCD19(+) cases (P < .001). We validated expression using flow cytometry and tissue microarray and demonstrated a 5.6-fold increase of TOSO protein in circulating CLL cells (P = .013) and lymph nodes (P = .006). Our SAGE results have demonstrated that TOSO is a novel over-expressed antiapoptotic gene in CLL.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-10477712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-11733577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-11733578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-12434020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-12522010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-14595126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-14726163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-15048066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-15161673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-15329427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-15459216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-15626485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-15632112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-15802535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-16121219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-16181674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-16950856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-17033038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-17804543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-18032710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-18160666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-18160669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18434611-9586636
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1528-0020
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pubmed:author |
pubmed-author:CaretaFrancisco PFP,
pubmed-author:ClearAndrewA,
pubmed-author:FalcãoRoberto PRP,
pubmed-author:GribbenJohn GJG,
pubmed-author:LeeAbigailA,
pubmed-author:MorrisKellyK,
pubmed-author:OwenCarolynC,
pubmed-author:PanepucciRodrigo ARA,
pubmed-author:Proto-SiqueiraRodrigoR,
pubmed-author:RizzattiEdgar GEG,
pubmed-author:SilvaWilson AWAJr,
pubmed-author:ZagoMarco AMA
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
112
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
394-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:18434611-Antigens, CD95,
pubmed-meshheading:18434611-Apoptosis Regulatory Proteins,
pubmed-meshheading:18434611-Gene Expression Profiling,
pubmed-meshheading:18434611-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:18434611-Humans,
pubmed-meshheading:18434611-Leukemia, Lymphocytic, Chronic, B-Cell,
pubmed-meshheading:18434611-Membrane Proteins
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pubmed:year |
2008
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pubmed:articleTitle |
SAGE analysis demonstrates increased expression of TOSO contributing to Fas-mediated resistance in CLL.
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pubmed:affiliation |
Hematology Division and Center for Cell-Based Therapy, Faculty of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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