Linked Life Data

18433621

Source:http://linkedlifedata.com/resource/pubmed/id/18433621

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-4-24
pubmed:abstractText
The flavodiiron proteins (FDPs), present in Archaea, Bacteria, and some protozoan pathogens (mostly anaerobes or microaerophiles), have been proposed to afford protection to microbes against nitric oxide and/or oxygen (toxic for anaerobes). The structural prototype of this protein family is a homodimer assembled in a "head-to-tail" configuration, with each monomer being composed of two domains: an N-terminal metallo-beta-lactamase module harboring a nonheme diiron center (active site of NO/O(2) reduction) and a C-terminal flavodoxin module, where a flavin mononucleotide moiety is embedded. Several FDPs bear C-terminal extra domains, which influence the composition of the respective electron transfer chains that couple NAD(P)H oxidation to NO/O(2) reduction. Herein are described methodologies employed to successfully produce, isolate, and characterize fully operative recombinant flavodiiron proteins. Spectroscopic techniques, namely absorption (visible and near-ultraviolet) and electron paramagnetic resonance spectroscopies, allowed redox-sensitive spectral fingerprints to be obtained, used further in the functional characterization of isolated flavodiiron proteins. Altogether, these studies on pure proteins contribute to understanding the molecular determinants that govern the in vivo function of the FDPs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0076-6879
pubmed:author
pubmed:issnType
Print
pubmed:volume
437
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21-45
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Biochemical, spectroscopic, and thermodynamic properties of flavodiiron proteins.
pubmed:affiliation
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't